Dataset Information

Myelin water imaging depends on white matter fiber orientation in the human brain.

ABSTRACT:

Purpose

The multi-exponential T₂ decay of the MRI signal from cerebral white matter can be separated into short T₂ components related to myelin water and long T₂ components related to intracellular and extracellular water. In this study, we investigated to what degree the apparent myelin water fraction (MWF) depends on the angle between white matter fibers and the main magnetic field.

Methods

Maps of the apparent MWF were acquired using multi-echo Carr-Purcell-Meiboom-Gill and gradient-echo spin-echo sequences. The Carr-Purcell-Meiboom-Gill sequence was acquired with a TR of 1073 ms, 1500 ms, and 2000 ms. The fiber orientation was mapped with DTI. By angle-wise pooling the voxels across the brain's white matter, orientation-dependent apparent MWF curves were generated.

Results

We found that the apparent MWF varied between 25% and 35% across different fiber orientations. Furthermore, the selection of the TR influences the apparent MWF.

Conclusion

White matter fiber orientation induces a strong systematic bias on the estimation of the apparent MWF. This finding has implications for future research and the interpretation of MWI results in previously published studies.

SUBMITTER: Birkl C

PROVIDER: S-EPMC7821018 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature

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Publications

Myelin water imaging depends on white matter fiber orientation in the human brain.

Birkl Christoph C Doucette Jonathan J Fan Michael M Hernández-Torres Enedino E Rauscher Alexander A

Magnetic resonance in medicine 20201005 4

<h4>Purpose</h4>The multi-exponential T<sub>2</sub> decay of the MRI signal from cerebral white matter can be separated into short T<sub>2</sub> components related to myelin water and long T<sub>2</sub> components related to intracellular and extracellular water. In this study, we investigated to what degree the apparent myelin water fraction (MWF) depends on the angle between white matter fibers and the main magnetic field.<h4>Methods</h4>Maps of the apparent MWF were acquired using multi-echo ...[more]

PMID: 33017486

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Project description:Diffusion MRI (dMRI) represents one of the few methods for mapping brain fiber orientations non-invasively. Unfortunately, dMRI fiber mapping is an indirect method that relies on inference from measured diffusion patterns. Comparing dMRI results with other modalities is a way to improve the interpretation of dMRI data and help advance dMRI technologies. Here, we present methods for comparing dMRI fiber orientation estimates with optical imaging of fluorescently labeled neurofilaments and vasculature in 3D human and primate brain tissue cuboids cleared using CLARITY. The recent advancements in tissue clearing provide a new opportunity to histologically map fibers projecting in 3D, which represents a captivating complement to dMRI measurements. In this work, we demonstrate the capability to directly compare dMRI and CLARITY in the same human brain tissue and assess multiple approaches for extracting fiber orientation estimates from CLARITY data. We estimate the three-dimensional neuronal fiber and vasculature orientations from neurofilament and vasculature stained CLARITY images by calculating the tertiary eigenvector of structure tensors. We then extend CLARITY orientation estimates to an orientation distribution function (ODF) formalism by summing multiple sub-voxel structure tensor orientation estimates. In a sample containing part of the human thalamus, there is a mean angular difference of 19o±15o between the primary eigenvectors of the dMRI tensors and the tertiary eigenvectors from the CLARITY neurofilament stain. We also demonstrate evidence that vascular compartments do not affect the dMRI orientation estimates by showing an apparent lack of correspondence (mean angular difference = 49o±23o) between the orientation of the dMRI tensors and the structure tensors in the vasculature stained CLARITY images. In a macaque brain dataset, we examine how the CLARITY feature extraction depends on the chosen feature extraction parameters. By varying the volume of tissue over which the structure tensor estimates are derived, we show that orientation estimates are noisier with more spurious ODF peaks for sub-voxels below 30 µm3 and that, for our data, the optimal gray matter sub-voxel size is between 62.5 µm3 and 125 µm3. The example experiments presented here represent an important advancement towards robust multi-modal MRI-CLARITY comparisons.

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Dataset Information

Myelin water imaging depends on white matter fiber orientation in the human brain.

Purpose

Methods

Results

Conclusion

Publications

Myelin water imaging depends on white matter fiber orientation in the human brain.

Similar Datasets

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