MiR?653?5p suppresses the growth and migration of breast cancer cells by targeting MAPK6.

ABSTRACT: Breast cancer is the worldwide leading cause of cancer?related deaths among women. Increasing evidence has demonstrated that microRNAs (miRNAs) play critical roles in the carcinogenesis and progression of breast cancer. miR?653?5p was previously reported to be involved in cell proliferation and apoptosis. However, the role of miR?653?5p in the progression of breast cancer has not been studied. In the present study, it was found that overexpression of miR?653?5p significantly inhibited the proliferation, migration and invasion of breast cancer cells in vitro. Moreover, overexpression of miR?653?5p promoted cell apoptosis in breast cancer by regulating the Bcl?2/Bax axis and caspase?9 activation. Additionally, the epithelial?mesenchymal transition and activation of the Akt/mammalian target of rapamycin signaling pathway were also inhibited by miR?653?5p. Furthermore, the data demonstrated that miR?653?5p directly targeted mitogen?activated protein kinase 6 (MAPK6) and negatively regulated its expression in breast cancer cells. Upregulation of MAPK6 could overcome the inhibitory effects of miR?653?5p on cell proliferation and migration in breast cancer. In conclusion, this study suggested that miR?653?5p functions as a tumor suppressor by targeting MAPK6 in the progression of breast cancer, and it may be a potential target for breast cancer therapy.

SUBMITTER: Zhang M 

PROVIDER: S-EPMC7821282 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature