Biomechanical Properties of Blood Plasma Extracellular Vesicles Revealed by Atomic Force Microscopy.

ABSTRACT: While extracellular vesicles (EVs) are extensively studied by various practical applications in biomedicine, there is still little information on their biomechanical properties due to their nanoscale size. We identified isolated blood plasma vesicles that carried on biomarkers associated with exosomes and exomeres and applied atomic force microscopy (AFM) to study them at single particle level in air and in liquid. Air measurements of exosomes revealed a mechanically indented internal cavity in which highly adhesive sites were located. In contrast, the highly adhesive sites of exomeres were located at the periphery and the observed diameter of the particles was ~35 nm. In liquid, the reversible deformation of the internal cavity of exosomes was observed and a slightly deformed lipid bi-lay

SUBMITTER: Bairamukov V 

PROVIDER: S-EPMC7822188 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature