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LINE-1 retrotransposon methylation in chorionic villi of first trimester miscarriages with aneuploidy.


ABSTRACT:

Purpose

High frequency of aneuploidy in meiosis and cleavage stage coincides with waves of epigenetic genome reprogramming that may indicate a possible association between epigenetic mechanisms and aneuploidy occurrence. This study aimed to assess the methylation level of the long interspersed repeat element 1 (LINE-1) retrotransposon in chorionic villi of first trimester miscarriages with a normal karyotype and aneuploidy.

Methods

The methylation level was assessed at 19 LINE-1 promoter CpG sites in chorionic villi of 141 miscarriages with trisomy of chromosomes 2, 6, 8-10, 13-15, 16, 18, 20-22, and monosomy X using massive parallel sequencing.

Results

The LINE-1 methylation level was elevated statistically significant in chorionic villi of miscarriages with both trisomy (45.2 ± 4.3%) and monosomy X (46.9 ± 4.2%) compared with that in induced abortions (40.0 ± 2.4%) (p < 0.00001). The LINE-1 methylation levels were specific for miscarriages with different aneuploidies and significantly increased in miscarriages with trisomies 8, 14, and 18 and monosomy X (p < 0.05). The LINE-1 methylation level increased with gestational age both for group of miscarriages regardless of karyotype (R = 0.21, p = 0.012) and specifically for miscarriages with trisomy 16 (R = 0.48, p = 0.007). LINE-1 methylation decreased with maternal age in miscarriages with a normal karyotype (R = - 0.31, p = 0.029) and with trisomy 21 (R = - 0.64, p = 0.024) and increased with paternal age for miscarriages with trisomy 16 (R = 0.38, p = 0.048) and monosomy X (R = 0.73, p = 0.003).

Conclusion

Our results indicate that the pathogenic effects of aneuploidy in human embryogenesis can be supplemented with significant epigenetic changes in the repetitive sequences.

SUBMITTER: Vasilyev SA 

PROVIDER: S-EPMC7823001 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature

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Publications

LINE-1 retrotransposon methylation in chorionic villi of first trimester miscarriages with aneuploidy.

Vasilyev Stanislav A SA   Tolmacheva Ekaterina N EN   Vasilyeva Oksana Yu OY   Markov Anton V AV   Zhigalina Daria I DI   Zatula Lada A LA   Lee Vasilissa A VA   Serdyukova Ekaterina S ES   Sazhenova Elena A EA   Nikitina Tatyana V TV   Kashevarova Anna A AA   Lebedev Igor N IN  

Journal of assisted reproduction and genetics 20201110 1


<h4>Purpose</h4>High frequency of aneuploidy in meiosis and cleavage stage coincides with waves of epigenetic genome reprogramming that may indicate a possible association between epigenetic mechanisms and aneuploidy occurrence. This study aimed to assess the methylation level of the long interspersed repeat element 1 (LINE-1) retrotransposon in chorionic villi of first trimester miscarriages with a normal karyotype and aneuploidy.<h4>Methods</h4>The methylation level was assessed at 19 LINE-1 p  ...[more]

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