Dataset Information

Restricted TcR ? chain CDR3 clonotype is associated with resolved acute hepatitis B subjects.

ABSTRACT:

Background

T cells play an important role in the prognosis of hepatitis B virus (HBV) infection, and are involved in the seroconversion of a patient from HBsAb negative to positive. To compare the T-cell receptor ?-chain variable region (TcRBV) complementarity-determining region 3 (CDR3) in subjects with or without hepatitis B surface antigen (HBsAg) convert to hepatitis B surface antibody (HBsAb), the TcRBV was determined using high throughput sequencing (HTS).

Methods

The clonotype and diversity of CDR3 in peripheral blood mononuclear cells of subjects with resolved acute hepatitis B (AHB, HBsAb+, HBsAg-) (n?=?5), chronic hepatitis B (CHB, HBsAb-, HBsAg+) (n?=?5), and healthy controls (HC, HBsAb-, HBsAg-) (n?=?3) were determined and analyzed using HTS (MiSeq).

Results

The overlapping rate of CDR3 clones of any two samples in AHB group was 2.00% (1.74%?~?2.30%), CHB group was 1.77% (1.43%?~?2.61%), and HC group was 1.82% (1.62%?~?2.12%), and there was no significant difference among the three groups by Kruskal-Wallis H test. However, among the top 10 cumulative frequencies of clonotypes, only the frequency of clonotype (TcRBV20-1/BD1/BJ1-2) in AHB group was lower than that of HC group (P??0.05).

Conclusions

Thus, there are 57 TcRBV clonotypes that may be related to HBsAg seroconversion of AHB subjects, but the diversity of TcRBV CDR3 is not significantly related to the HBsAb positive status.

SUBMITTER: Xiao D

PROVIDER: S-EPMC7825183 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature

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Publications

Restricted TcR β chain CDR3 clonotype is associated with resolved acute hepatitis B subjects.

Xiao Dangsheng D Wang Ju J Chen Zhitao Z Jin Xiuyuan X Xie Yirui Y Yan Dong D Yang Jiezuan J

BMC infectious diseases 20210123 1

<h4>Background</h4>T cells play an important role in the prognosis of hepatitis B virus (HBV) infection, and are involved in the seroconversion of a patient from HBsAb negative to positive. To compare the T-cell receptor β-chain variable region (TcRBV) complementarity-determining region 3 (CDR3) in subjects with or without hepatitis B surface antigen (HBsAg) convert to hepatitis B surface antibody (HBsAb), the TcRBV was determined using high throughput sequencing (HTS).<h4>Methods</h4>The clonot ...[more]

PMID: 33485302

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