Dataset Information

Genomic Characterization of VIM and MCR Co-Producers: The First Two Clinical Cases, in Italy.

ABSTRACT:

Background

the co-production of carbapenemases and mcr-genes represents a worrisome event in the treatment of Enterobacteriaceae infections. The aim of the study was to characterize the genomic features of two clinical Enterobacter cloacae complex (ECC) isolates, co-producing VIM and MCR enzymes, in Italy.

Methods

species identification and antibiotic susceptibility profiling were performed using MALDI-TOF and broth microdilution methods, respectively. Transferability of the bla_VIM- and mcr- type genes was verified through conjugation experiment. Extracted DNA was sequenced using long reads sequencing technology on the Sequel I platform (PacBio).

Results

the first isolate showed clinical resistance against ertapenem yet was colistin susceptible (EUCAST 2020 breakpoints). The mcr-9.2 gene was harbored on a conjugative IncHI2 plasmid, while the bla_VIM-1 determinant was harbored on a conjugative IncN plasmid. The second isolate, resistant to both carbapenems and colistin, harbored: mcr-9 gene and its two component regulatory genes for increased expression on the chromosome, mcr-4.3 on non-conjugative (yet co-transferable) ColE plasmid, and bla_VIM-1 on a non-conjugative IncA plasmid.

Conclusions

to our knowledge, this is the first report of co-production of VIM and MCR in ECC isolates in Italy.

SUBMITTER: Marchetti VM

PROVIDER: S-EPMC7825325 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Genomic Characterization of VIM and MCR Co-Producers: The First Two Clinical Cases, in Italy.

Marchetti Vittoria Mattioni VM Bitar Ibrahim I Sarti Mario M Fogato Elena E Scaltriti Erika E Bracchi Chiara C Hrabak Jaroslav J Pongolini Stefano S Migliavacca Roberta R

Diagnostics (Basel, Switzerland) 20210106 1

<h4>Background</h4>the co-production of carbapenemases and <i>mcr</i>-genes represents a worrisome event in the treatment of <i>Enterobacteriaceae</i> infections. The aim of the study was to characterize the genomic features of two clinical <i>Enterobacter cloacae</i> complex (ECC) isolates, co-producing VIM and MCR enzymes, in Italy.<h4>Methods</h4>species identification and antibiotic susceptibility profiling were performed using MALDI-TOF and broth microdilution methods, respectively. Transfe ...[more]

PMID: 33418979

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Dataset Information

Genomic Characterization of VIM and MCR Co-Producers: The First Two Clinical Cases, in Italy.

Background

Methods

Results

Conclusions

Publications

Genomic Characterization of VIM and MCR Co-Producers: The First Two Clinical Cases, in Italy.

Similar Datasets

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