Dataset Information

N-Glycosylation in Piroplasmids: Diversity within Simplicity.

ABSTRACT: N-glycosylation has remained mostly unexplored in Piroplasmida, an order of tick-transmitted pathogens of veterinary and medical relevance. Analysis of 11 piroplasmid genomes revealed three distinct scenarios regarding N-glycosylation: Babesia sensu stricto (s.s.) species add one or two N-acetylglucosamine (NAcGlc) molecules to proteins; Theileria equi and Cytauxzoon felis add (NAcGlc)₂-mannose, while B. microti and Theileria s.s. synthesize dolichol-P-P-NAcGlc and dolichol-P-P-(NAcGlc)₂ without subsequent transfer to proteins. All piroplasmids possess the gene complement needed for the synthesis of the N-glycosylation substrates, dolichol-P and sugar nucleotides. The oligosaccharyl transferase of Babesia species, T. equi and C. felis, is predicted to be composed of only two subunits, STT3 and Ost1. Occurrence of short N-glycans in B. bovis merozoites was experimentally demonstrated by fluorescence microscopy using a NAcGlc-specific lectin. In vitro growth of B. bovis was significantly impaired by tunicamycin, an inhibitor of N-glycosylation, indicating a relevant role for N-glycosylation in this pathogen. Finally, genes coding for N-glycosylation enzymes and substrate biosynthesis are transcribed in B. bovis blood and tick stages, suggesting that this pathway is biologically relevant throughout the parasite life cycle. Elucidation of the role/s exerted by N-glycans will increase our understanding of these successful parasites, for which improved control measures are needed.

SUBMITTER: Florin-Christensen M

PROVIDER: S-EPMC7826898 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature

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N-Glycosylation in Piroplasmids: Diversity within Simplicity.

Florin-Christensen Monica M Rodriguez Anabel E AE Suárez Carlos E CE Ueti Massaro W MW Delgado Fernando O FO Echaide Ignacio I Schnittger Leonhard L

Pathogens (Basel, Switzerland) 20210108 1

N-glycosylation has remained mostly unexplored in Piroplasmida, an order of tick-transmitted pathogens of veterinary and medical relevance. Analysis of 11 piroplasmid genomes revealed three distinct scenarios regarding N-glycosylation: Babesia sensu stricto (s.s.) species add one or two N-acetylglucosamine (NAcGlc) molecules to proteins; Theileria equi and Cytauxzoon felis add (NAcGlc)2-mannose, while B. microti and Theileria s.s ...[more]

PMID: 33429911

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Dataset Information

N-Glycosylation in Piroplasmids: Diversity within Simplicity.

Publications

<i>N</i>-Glycosylation in Piroplasmids: Diversity within Simplicity.

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