Unknown

Dataset Information

0

SARS-CoV-2 Epitope Mapping on Microarrays Highlights Strong Immune-Response to N Protein Region.


ABSTRACT: A workflow for rapid SARS-CoV-2 epitope discovery on peptide microarrays is herein reported. The process started with a proteome-wide screening of immunoreactivity based on the use of a high-density microarray followed by a refinement and validation phase on a restricted panel of probes using microarrays with tailored peptide immobilization through a click-based strategy. Progressively larger, independent cohorts of Covid-19 positive sera were tested in the refinement processes, leading to the identification of immunodominant regions on SARS-CoV-2 spike (S), nucleocapsid (N) protein and Orf1ab polyprotein. A summary study testing 50 serum samples highlighted an epitope of the N protein (region 155-71) providing good diagnostic performance in discriminating Covid-19 positive vs. healthy individuals. Using this epitope, 92% sensitivity and 100% specificity were reached for IgG detection in Covid-19 samples, and no cross-reactivity with common cold coronaviruses was detected. Likewise, IgM immunoreactivity in samples collected within the first month after symptoms onset showed discrimination ability. Overall, epitope 155-171 from N protein represents a promising candidate for further development and rapid implementation in serological tests.

SUBMITTER: Musico A 

PROVIDER: S-EPMC7827214 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature

altmetric image

Publications


A workflow for rapid SARS-CoV-2 epitope discovery on peptide microarrays is herein reported. The process started with a proteome-wide screening of immunoreactivity based on the use of a high-density microarray followed by a refinement and validation phase on a restricted panel of probes using microarrays with tailored peptide immobilization through a click-based strategy. Progressively larger, independent cohorts of Covid-19 positive sera were tested in the refinement processes, leading to the i  ...[more]

Similar Datasets

2023-08-31 | E-MTAB-13040 | biostudies-arrayexpress
| S-SCDT-EMBOR-2021-54341-T | biostudies-other
| S-SCDT-EMBOR-2020-51252V1 | biostudies-other
| S-EPMC7568776 | biostudies-literature
| S-EPMC7522676 | biostudies-literature
| S-EPMC7599751 | biostudies-literature
| S-EPMC7605265 | biostudies-literature
| S-EPMC2762542 | biostudies-other
| S-EPMC7494247 | biostudies-literature
| S-EPMC8065321 | biostudies-literature