Unknown

Dataset Information

0

Novel Extracellular Electron Transfer Channels in a Gram-Positive Thermophilic Bacterium.


ABSTRACT: Biogenic transformation of Fe minerals, associated with extracellular electron transfer (EET), allows microorganisms to exploit high-potential refractory electron acceptors for energy generation. EET-capable thermophiles are dominated by hyperthermophilic archaea and Gram-positive bacteria. Information on their EET pathways is sparse. Here, we describe EET channels in the thermophilic Gram-positive bacterium Carboxydothermus ferrireducens that drive exoelectrogenesis and rapid conversion of amorphous mineral ferrihydrite to large magnetite crystals. Microscopic studies indicated biocontrolled formation of unusual formicary-like ultrastructure of the magnetite crystals and revealed active colonization of anodes in bioelectrochemical systems (BESs) by C. ferrireducens. The internal structure of micron-scale biogenic magnetite crystals is reported for the first time. Genome analysis and expression profiling revealed three constitutive c-type multiheme cytochromes involved in electron exchange with ferrihydrite or an anode, sharing insignificant homology with previously described EET-related cytochromes thus representing novel determinants of EET. Our studies identify these cytochromes as extracellular and reveal potentially novel mechanisms of cell-to-mineral interactions in thermal environments.

SUBMITTER: Gavrilov SN 

PROVIDER: S-EPMC7829351 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

altmetric image

Publications


Biogenic transformation of Fe minerals, associated with extracellular electron transfer (EET), allows microorganisms to exploit high-potential refractory electron acceptors for energy generation. EET-capable thermophiles are dominated by hyperthermophilic archaea and Gram-positive bacteria. Information on their EET pathways is sparse. Here, we describe EET channels in the thermophilic Gram-positive bacterium <i>Carboxydothermus ferrireducens</i> that drive exoelectrogenesis and rapid conversion  ...[more]

Similar Datasets

| S-EPMC6703420 | biostudies-literature
| S-EPMC7969598 | biostudies-literature
| S-EPMC6936397 | biostudies-literature
| S-EPMC6221200 | biostudies-literature
| S-EPMC3209153 | biostudies-literature
2021-09-09 | PXD018523 | Pride
| S-EPMC8027190 | biostudies-literature
| S-EPMC4142287 | biostudies-literature
| S-EPMC8316523 | biostudies-literature
| S-EPMC5808234 | biostudies-literature