Blood transcriptomics to characterize key biological pathways and identify biomarkers for predicting mortality in melioidosis.

ABSTRACT: Melioidosis is an often lethal tropical disease caused by the Gram-negative bacillus, Burkholderia pseudomallei. The study objective was to characterize transcriptomes in melioidosis patients and identify genes associated with outcome. Whole blood RNA-seq was performed in a discovery set of 29 melioidosis patients and 3 healthy controls. Transcriptomic profiles of patients who did not survive to 28 days were compared with patients who survived and healthy controls, showing 65 genes were significantly up-regulated and 218 were down-regulated in non-survivors compared to survivors. Up-regulated genes were involved in myeloid leukocyte activation, Toll-like receptor cascades and reactive oxygen species metabolic processes. Down-regulated genes were hematopoietic cell lineage, adaptive

SUBMITTER: Yimthin T 

PROVIDER: S-EPMC7832033 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature