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In Vitro Interactions of TiO2 Nanoparticles with Earthworm Coelomocytes: Immunotoxicity Assessment.


ABSTRACT: Titanium dioxide nanoparticles (TiO2 NPs) are manufactured worldwide. Once they arrive in the soil environment, they can endanger living organisms. Hence, monitoring and assessing the effects of these nanoparticles is required. We focus on the Eisenia andrei earthworm immune cells exposed to sublethal concentrations of TiO2 NPs (1, 10, and 100 µg/mL) for 2, 6, and 24 h. TiO2 NPs at all concentrations did not affect cell viability. Further, TiO2 NPs did not cause changes in reactive oxygen species (ROS) production, malondialdehyde (MDA) production, and phagocytic activity. Similarly, they did not elicit DNA damage. Overall, we did not detect any toxic effects of TiO2 NPs at the cellular level. At the gene expression level, slight changes were detected. Metallothionein, fetidin/lysenin, lumbricin and MEK kinase I were upregulated in coelomocytes after exposure to 10 µg/mL TiO2 NPs for 6 h. Antioxidant enzyme expression was similar in exposed and control cells. TiO2 NPs were detected on coelomocyte membranes. However, our results do not show any strong effects of these nanoparticles on coelomocytes at both the cellular and molecular levels.

SUBMITTER: Navarro Pacheco NI 

PROVIDER: S-EPMC7832855 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature

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In Vitro Interactions of TiO<sub>2</sub> Nanoparticles with Earthworm Coelomocytes: Immunotoxicity Assessment.

Navarro Pacheco Natividad Isabel NI   Roubalova Radka R   Semerad Jaroslav J   Grasserova Alena A   Benada Oldrich O   Kofronova Olga O   Cajthaml Tomas T   Dvorak Jiri J   Bilej Martin M   Prochazkova Petra P  

Nanomaterials (Basel, Switzerland) 20210119 1


Titanium dioxide nanoparticles (TiO<sub>2</sub> NPs) are manufactured worldwide. Once they arrive in the soil environment, they can endanger living organisms. Hence, monitoring and assessing the effects of these nanoparticles is required. We focus on the <i>Eisenia andrei</i> earthworm immune cells exposed to sublethal concentrations of TiO<sub>2</sub> NPs (1, 10, and 100 µg/mL) for 2, 6, and 24 h. TiO<sub>2</sub> NPs at all concentrations did not affect cell viability. Further, TiO<sub>2</sub>  ...[more]

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