Increased [¹⁸F]FMISO accumulation under hypoxia by multidrug-resistant protein 1 inhibitors.

ABSTRACT:

Background

[¹⁸F]Fluoromisonidazole ([¹⁸F]FMISO) is a PET imaging probe widely used for the detection of hypoxia. We previously reported that [¹⁸F]FMISO is metabolized to the glutathione conjugate of the reduced form in hypoxic cells. In addition, we found that the [¹⁸F]FMISO uptake level varied depending on the cellular glutathione conjugation and excretion ability such as enzyme activity of glutathione-S-transferase and expression levels of multidrug resistance-associated protein 1 (MRP1, an efflux transporter), in addition to the cellular hypoxic state. In this study, we evaluated whether MRP1 activity affected [¹⁸F]FMISO PET imaging.

Methods

FaDu human pharyngeal squamous cell carcinoma cells were pretreated with MRP1

SUBMITTER: Shimizu Y 

PROVIDER: S-EPMC7835267 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature