Assembling Surface Linker Chemistry with Minimization of Non-Specific Adsorption on Biosensor Materials.

ABSTRACT: The operation of biosensors requires surfaces that are both highly specific towards the target analyte and that are minimally subject to fouling by species present in a biological fluid. In this work, we further examined the thiosulfonate-based linker in order to construct robust and durable self-assembling monolayers (SAMs) onto hydroxylated surfaces such as silica. These SAMs are capable of the chemoselective immobilization of thiol-containing probes (for analytes) under aqueous conditions in a single, straightforward, reliable, and coupling-free manner. The efficacy of the method was assessed through implementation as a biosensing interface for an ultra-high frequency acoustic wave device dedicated to the detection of avidin via attached biotin. Fouling was assessed via introduction of

SUBMITTER: Sheng JC 

PROVIDER: S-EPMC7835736 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature