Dataset Information

Prediction of Calving to Conception Interval Length Using Algorithmic Analysis of Endometrial mRNA Expression in Bovine.

ABSTRACT: After parturition, the uterus undergoes significant reconstruction, allows the endometrium to create an environment for subsequent embryo development. Here, we used an unsupervised algorithmic approach to select characteristic endometrial mRNA expression patterns of proposed markers and investigate each marker's role as an individual indicator of reproductive success. Clinically healthy cows at a sixth week postpartum were examined, the percentage of neutrophils (PMNs%) in the cytological smear was calculated, and an endometrial biopsy was taken for qPCR. Based on pregnancy examination, cows were divided into three groups: Pregnant before 100 days postpartum (P100, n = 11), pregnant between 100-200-day (P200, n = 14), and culled (C, n = 10). Animals were also classified based on two PMNs% thresholds > 5% PMNs and > 10% PMNs. The expression of IL1B, IL6, CXCL8, and IL17A was higher in >10%PMNs. The expression of PTGS1 was higher in the P200 compared to P100. Upregulation of inhibin A subunit (INHA) and downregulation of inhibin ? A subunit (INHBA) were observed in the P100. INHBA/INHA ratio was the most accurate linear predictor of the calving-to-conception interval. The application of the k-means algorithm allowed the identification of five unique expression patterns. The sensitivity and specificity of predicting allocation to P100 were 81% and 79%. We also documented the low efficiency of genes associated with subclinical endometritis and PMNs% in determining reproductive capability. These results suggested the presence of distinctive expression patterns in 6 weeks postpartum, correlated with cows' reproductive capacity. Furthermore, we proposed the INHBA/INHA ratio as an indicator of calving-to-conception interval length.

SUBMITTER: Tobolski D

PROVIDER: S-EPMC7835770 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature

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Prediction of Calving to Conception Interval Length Using Algorithmic Analysis of Endometrial mRNA Expression in Bovine.

Tobolski Dawid D Łukasik Karolina K Bacławska Agnieszka A Skarżyński Dariusz Jan DJ Hostens Miel M Barański Wojciech W

Animals : an open access journal from MDPI 20210119 1

After parturition, the uterus undergoes significant reconstruction, allows the endometrium to create an environment for subsequent embryo development. Here, we used an unsupervised algorithmic approach to select characteristic endometrial mRNA expression patterns of proposed markers and investigate each marker's role as an individual indicator of reproductive success. Clinically healthy cows at a sixth week postpartum were examined, the percentage of neutrophils (PMNs%) in the cytological smear ...[more]

PMID: 33478124

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Project description:Prolonged calving-to-conception interval (CCI) can increase economic loss in cattle. We investigated the effect of post-calving abnormal reproductive tract discharge (ARTD) on CCI in dairy cows and quantified the relationship of ARTD and associated risk factors with CCI. The source population was dairy cows that calved in the study period on three pasture-based, year-round calving farms in the Riverina, NSW, Australia. Farm records and records from veterinarians' visits were analyzed. ARTD was defined as the presence of reproductive tract discharge according to the following classification: per vaginum purulent discharge ?21 days post-calving or mucopurulent discharge >26 days post-calving. The incidence of ARTD was calculated. A Kaplan-Meier survivor function was used to estimate median time to conception post-calving dependent on the presence or absence of ARTD. Mixed effects Cox-proportional hazard models were used to estimate the direct and indirect effects of ARTD, and other potential risk factors on CCI such as body condition score (BCS), ambient temperature, and milk yield. Model structures were guided by a directed acyclic graph of potential risk factors for ARTD. The incidence of ARTD in lactations was 16% (95% CI 13.8-18.5%) and did not differ significantly between the three farms (P > 0.05). The median CCI was 176 and 118 days for lactations with and without ARTD, respectively (P < 0.01). The rate of pregnancy following calving in cows with ARTD was significantly decreased relative to the rate of pregnancy in cows without (total effect hazard ratio = 0.62, se = 0.18, P = 0.01). High peak milk yield (>32 L) and parity >2 also significantly extended CCI. We did not observe an effect of BCS or ambient temperature on CCI. The incidence of ARTD in the current study was consistent with clinical endometritis (considered a major source of ARTD) reported in other studies. In contrast-and despite regular veterinary assessment and treatment of on the farms in this study-ARTD extended CCI. Evaluation of the economic impact of ARTD on dairy farms in this region is warranted, and methods to identify high risk cows and develop effective interventions are required.

Dataset Information

Prediction of Calving to Conception Interval Length Using Algorithmic Analysis of Endometrial mRNA Expression in Bovine.

Publications

Prediction of Calving to Conception Interval Length Using Algorithmic Analysis of Endometrial mRNA Expression in Bovine.

Similar Datasets

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