Ontology highlight
ABSTRACT: Background and aims
Immediate detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is critical for preventing the spread of coronavirus disease 2019 (COVID-19). The LabTurbo AIO 48 system is an automated platform that allows nucleic acid extraction and sample analysis on the same instrument, producing faster results without affecting their accuracy. We aimed to independently evaluate the LabTurbo AIO 48 (all-in-one system) for SARS-CoV-2 detection.Materials and methods
Comparative limit of detection (LOD) was assessed on both the LabTurbo AIO 48 and current standard detection system based on real-time reverse transcriptase polymerase chain reaction (RT-PCR), using SARS-CoV-2 RNA control. Additional 125 primary clinical samples were assessed using both the protocols in parallel.Results
The turnaround time from sample to results for 48 samples analyzed on LabTurbo AIO 48 was approximately 2.5 h, whereas that analyzed using the in-house RT-PCR protocol was 4.8 h. LabTurbo AIO 48 also demonstrated higher sensitivity than our reference RT-PCR assay, with a LOD of 9.4 copies/reaction. The overall percentage agreement between both the methods for 125 samples was 100%.Conclusion
LabTurbo AIO 48 is a robust detection option for SARS-CoV-2, allowing faster results and, consequently, aiding in better control and prevention of COVID-19.
SUBMITTER: Jian MJ
PROVIDER: S-EPMC7836538 | biostudies-literature | 2021 Mar
REPOSITORIES: biostudies-literature
Jian Ming-Jr MJ Chung Hsing-Yi HY Chang Chih-Kai CK Lin Jung-Chung JC Yeh Kuo-Ming KM Chiu Sheng-Kang SK Wang Yi-Hui YH Liao Shu-Jung SJ Li Shih-Yi SY Hsieh Shan-Shan SS Perng Cherng-Lih CL Chang Feng-Yee FY Shang Hung-Sheng HS
Clinica chimica acta; international journal of clinical chemistry 20201213
<h4>Background and aims</h4>Immediate detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is critical for preventing the spread of coronavirus disease 2019 (COVID-19). The LabTurbo AIO 48 system is an automated platform that allows nucleic acid extraction and sample analysis on the same instrument, producing faster results without affecting their accuracy. We aimed to independently evaluate the LabTurbo AIO 48 (all-in-one system) for SARS-CoV-2 detection.<h4>Materials and m ...[more]