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Synthesis and Evaluation of Europium Complexes that Switch on Luminescence in Lysosomes of Living Cells.

ABSTRACT: A set of four luminescent EuIII complexes bearing an extended aryl-alkynylpyridine chromophore has been studied, showing very different pH-dependent behaviour in their absorption and emission spectral response. For two complexes with pKa values of 6.45 and 6.20 in protein-containing solution, the emission lifetime increases very significantly following protonation. By varying the gate time during signal acquisition, the 'switch-on' intensity ratio could be optimised, and enhancement factors of between 250 to 1330 were measured between pH?8 and 4. The best-behaved probe showed no significant emission dependence on the concentration of endogenous cations, reductants, and serum albumin. It was examined in live-cell imaging studies to monitor time-dependent lysosomal acidification, for which the increase in observed image brightness due to acidification was a factor of 50 in NIH-3T3 cells.

SUBMITTER: Starck M 

PROVIDER: S-EPMC7839496 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature

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Synthesis and Evaluation of Europium Complexes that Switch on Luminescence in Lysosomes of Living Cells.

Starck Matthieu M   Fradgley Jack D JD   Pal Robert R   Zwier Jurriaan M JM   Lamarque Laurent L   Parker David D  

Chemistry (Weinheim an der Bergstrasse, Germany) 20201215 2

A set of four luminescent Eu<sup>III</sup> complexes bearing an extended aryl-alkynylpyridine chromophore has been studied, showing very different pH-dependent behaviour in their absorption and emission spectral response. For two complexes with pK<sub>a</sub> values of 6.45 and 6.20 in protein-containing solution, the emission lifetime increases very significantly following protonation. By varying the gate time during signal acquisition, the 'switch-on' intensity ratio could be optimised, and en  ...[more]

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