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Dmrt1 regulates the immune response by repressing the TLR4 signaling pathway in goat male germline stem cells.


ABSTRACT: Double sex and mab-3-related transcription factor 1 (Dmrt1), which is expressed in goat male germline stem cells (mGSCs) and Sertoli cells, is one of the most conserved transcription factors involved in sex determination. In this study, we highlighted the role of Dmrt1 in balancing the innate immune response in goat mGSCs. Dmrt1 recruited promyelocytic leukemia zinc finger (Plzf), also known as zinc finger and BTB domain-containing protein 16 (Zbtb16), to repress the Toll-like receptor 4 (TLR4)-dependent inflammatory signaling pathway and nuclear factor (NF)-?B. Knockdown of Dmrt1 in seminiferous tubules resulted in widespread degeneration of germ and somatic cells, while the expression of proinflammatory factors were significantly enhanced. We also demonstrated that Dmrt1 stimulated proliferation of mGSCs, but repressed apoptosis caused by the immune response. Thus, Dmrt1 is sufficient to reduce inflammation in the testes, thereby establishing the stability of spermatogenesis and the testicular microenvironment.

SUBMITTER: Wei YD 

PROVIDER: S-EPMC7840460 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature

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Dmrt1 regulates the immune response by repressing the TLR4 signaling pathway in goat male germline stem cells.

Wei Yu-Dong YD   Du Xiao-Min XM   Yang Dong-Hui DH   Ma Fang-Lin FL   Yu Xiu-Wei XW   Zhang Meng-Fei MF   Li Na N   Peng Sha S   Liao Ming-Zhi MZ   Li Guang-Peng GP   Bai Chun-Ling CL   Liu Wei-Shuai WS   Hua Jin-Lian JL  

Zoological research 20210101 1


Double sex and mab-3-related transcription factor 1 (Dmrt1), which is expressed in goat male germline stem cells (mGSCs) and Sertoli cells, is one of the most conserved transcription factors involved in sex determination. In this study, we highlighted the role of Dmrt1 in balancing the innate immune response in goat mGSCs. Dmrt1 recruited promyelocytic leukemia zinc finger (Plzf), also known as zinc finger and BTB domain-containing protein 16 (Zbtb16), to repress the Toll-like receptor 4 (TLR4)-  ...[more]

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