Dataset Information

Integrated Analysis of the Transcriptome and Metabolome Revealed the Molecular Mechanisms Underlying the Enhanced Salt Tolerance of Rice Due to the Application of Exogenous Melatonin.

ABSTRACT: High salinity is one of the major abiotic stresses limiting rice production. Melatonin has been implicated in the salt tolerance of rice. However, the molecular basis of melatonin-mediated salt tolerance in rice remains unclear. In the present study, we performed an integrated transcriptome and metabolome profiling of rice seedlings treated with salt, melatonin, or salt + melatonin. The application of exogenous melatonin increased the salt tolerance of rice plants by decreasing the sodium content to maintain Na⁺/K⁺ homeostasis, alleviating membrane lipid oxidation, and enhancing chlorophyll contention. A comparative transcriptome analysis revealed that complex molecular pathways contribute to melatonin-mediated salt tolerance. More specifically, the AP2/EREBP-HB-WRKY transcriptional cascade and phytohormone (e.g., auxin and abscisic acid) signaling pathways were activated by an exogenous melatonin treatment. On the basis of metabolome profiles, 64 metabolites, such as amino acids, organic acids, nucleotides, and secondary metabolites, were identified with increased abundances only in plants treated with salt + melatonin. Several of these metabolites including endogenous melatonin and its intermediates (5-hydroxy-L-tryptophan, N ¹-acetyl-N ²-formyl-5-methoxykynuramine), gallic acid, diosmetin, and cyanidin 3-O-galactoside had antioxidant functions, suggesting melatonin activates multiple antioxidant pathways to alleviate the detrimental effects of salt stress. Combined transcriptome and metabolome analyses revealed a few gene-metabolite networks related to various pathways, including linoleic acid metabolism and amino acid metabolism that are important for melatonin-mediated salt tolerance. The data presented herein may be useful for further elucidating the multiple regulatory roles of melatonin in plant responses to abiotic stresses.

SUBMITTER: Xie Z

PROVIDER: S-EPMC7840565 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Integrated Analysis of the Transcriptome and Metabolome Revealed the Molecular Mechanisms Underlying the Enhanced Salt Tolerance of Rice Due to the Application of Exogenous Melatonin.

Xie Ziyan Z Wang Juan J Wang Wensheng W Wang Yanru Y Xu Jianlong J Li Zhikang Z Zhao Xiuqin X Fu Binying B

Frontiers in plant science 20210114

High salinity is one of the major abiotic stresses limiting rice production. Melatonin has been implicated in the salt tolerance of rice. However, the molecular basis of melatonin-mediated salt tolerance in rice remains unclear. In the present study, we performed an integrated transcriptome and metabolome profiling of rice seedlings treated with salt, melatonin, or salt + melatonin. The application of exogenous melatonin increased the salt tolerance of rice plants by decreasing the sodium conten ...[more]

PMID: 33519878

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Project description:Due to global climate change, crops are certainly confronted with a lot of abiotic and biotic stress factors during their growth that cause a serious threat to their development and overall productivity. Among different abiotic stresses, salt and drought are considered the most devastating stressors with serious impact on crop's yield stability. Here, the current study aimed to elucidate how melatonin works in regulating plant biomass, oxidative stress, antioxidant defense system, as well as the expression of genes related to salt and drought stress in rice plants. Eight groups of rice plants (3 replicates, 5 plants each) underwent varied treatments: control, melatonin, salt, drought, salt + drought, salt + melatonin, drought + melatonin, and salt + drought + melatonin. Melatonin (100 µM) was alternately applied a week before stress exposure; salt stress received 100 mM NaCl every 3 days for 3 weeks, and drought stress involved 10% PEG. Young leaves were randomly sampled from each group. The results showed that melatonin treatment markedly reduces salt and drought stress damage by promoting root, shoot length, fresh and dry weight, increasing chlorophyll contents, and inhibiting excessive production of oxidative stress markers. Salt and drought stress significantly decreased the water balance, and damaged cell membrane by reducing relative water contents and increasing electrolyte leakage. However, melatonin treated rice plants showed high relative water contents and low electrolyte leakage. Under salt and drought stress conditions, exogenous application of melatonin boosted the expression level of salt and drought stress responsive genes like OsSOS, OsNHX, OsHSF and OsDREB in rice plants. Taken together, our results reveal that melatonin treatment significantly increases salt and drought tolerance of rice plants, by increasing plant biomass, suppressing ROS accumulation, elevating antioxidants defense efficiency, and up-regulating the expression of salt and drought stress responsive genes.

Dataset Information

Integrated Analysis of the Transcriptome and Metabolome Revealed the Molecular Mechanisms Underlying the Enhanced Salt Tolerance of Rice Due to the Application of Exogenous Melatonin.

Publications

Integrated Analysis of the Transcriptome and Metabolome Revealed the Molecular Mechanisms Underlying the Enhanced Salt Tolerance of Rice Due to the Application of Exogenous Melatonin.

Similar Datasets

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