Visualization of cortical microtubule networks in plant cells by live imaging and immunostaining.
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ABSTRACT:
SUBMITTER: Du F
PROVIDER: S-EPMC7843915 | biostudies-literature | 2021 Mar
REPOSITORIES: biostudies-literature
Publications
Visualization of cortical microtubule networks in plant cells by live imaging and immunostaining.
Du Fei F Zhao Feng F Traas Jan J Jiao Yuling Y
STAR protocols 20210123 1
Cortical microtubules (CMTs) play pivotal roles during plant cell growth and division. The organization of CMTs undergoes important changes during different cellular and developmental processes. Here, we describe two methods for the visualization of CMT organization in plant cells using confocal laser scanning microscopy. CMT networks in the outer tissue layers can be directly visualized by live imaging of a fluorescent reporter line, and a protocol combining sectioning and immunostaining is app ...[more]
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