Dataset Information

Ceftiofur reduced Fusobacterium leading to uterine microbiota alteration in dairy cows with metritis.

ABSTRACT:

Background

Metritis is an inflammatory uterine disease found in ~?20% of dairy cows after parturition and associated with uterine microbiota with high abundance of Fusobacterium, Bacteroides, and Porphyromonas. Ceftiofur is a common treatment, but the effect on uterine microbiota is poorly understood. Herein, we investigated the short-term impact of ceftiofur on uterine microbiota structure and function in cows with metritis. Eight cows received ceftiofur (CEF) and 10 remained untreated (CON). Uterine swabs were collected for PCR and metagenomic analysis at diagnosis before treatment (5 ± 1 DPP) and 2?days after diagnosis/treatment (7 ± 1 DPP) from the same individuals. Seven CEF and 9 CON passed quality control and were used for 16S rRNA gene sequencing.

Results

Ceftiofur treatment resulted in uterine microbiota alteration, which was attributed to a decrease in relative abundance of Fusobacterium and in gene contents involved in lipopolysaccharide biosynthesis, whereas uterine microbiota diversity and genes involved in pantothenate and coenzyme A biosynthesis increased. Ceftiofur treatment also reduced rectal temperature and tended to reduce total bacteria in the uterus. However, other uterine pathogens such as Bacteroides and Porphyromonas remained unchanged in CEF. The bla_CTX-M gene was detected in 37.5% of metritic cows tested but was not affected by CEF. We found that ?-hydroxybutyric acid, pyruvic acid, and L-glutamine were preferentially utilized by Fusobacterium necrophorum according to metabolic activity with 95 carbon sources.

Conclusions

Ceftiofur treatment leads to alterations in the uterine microbiota that were mainly characterized by reductions in Fusobacterium and genes involved in LPS biosynthesis, which may be associated with a decrease in rectal temperature. The increase in pantothenate and coenzyme A biosynthesis indicates microbial response to metabolic stress caused by ceftiofur. Preference of Fusobacterium for ?-hydroxybutyric acid may help to explain why this strain becomes dominant in the uterine microbiota of cows with metritis, and it also may provide a means for development of new therapies for the control of metritis in dairy cows.

SUBMITTER: Jeon SJ

PROVIDER: S-EPMC7844903 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature

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Publications

Ceftiofur reduced Fusobacterium leading to uterine microbiota alteration in dairy cows with metritis.

Jeon Soo Jin SJ Cunha Federico F Daetz Rodolfo R Bicalho Rodrigo C RC Lima Svetlana S Galvão Klibs N KN

Animal microbiome 20210128 1

<h4>Background</h4>Metritis is an inflammatory uterine disease found in ~ 20% of dairy cows after parturition and associated with uterine microbiota with high abundance of Fusobacterium, Bacteroides, and Porphyromonas. Ceftiofur is a common treatment, but the effect on uterine microbiota is poorly understood. Herein, we investigated the short-term impact of ceftiofur on uterine microbiota structure and function in cows with metritis. Eight cows received ceftiofur (CEF) and 10 remained untreated ...[more]

PMID: 33509303

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Dataset Information

Ceftiofur reduced Fusobacterium leading to uterine microbiota alteration in dairy cows with metritis.

Background

Results

Conclusions

Publications

Ceftiofur reduced Fusobacterium leading to uterine microbiota alteration in dairy cows with metritis.

Similar Datasets

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