Dataset Information

Change in the plasma proteome associated with canine cognitive dysfunction syndrome (CCDS) in Thailand.

ABSTRACT:

Background

Canine cognitive dysfunction syndrome (CCDS) is a progressive neurodegenerative disorder found in senior dogs. Due to the lack of biological markers, CCDS is commonly underdiagnosed. The aim of this study was to identify potential plasma biomarkers using proteomics techniques and to increase our understanding of the pathogenic mechanism of the disease. Plasma amyloid beta 42 (A?₄₂) has been seen to be a controversial biomarker for CCDS. Proteomics analysis was performed for protein identification and quantification.

Results

Within CCDS, ageing, and adult dogs, 87 proteins were identified specific to Canis spp. in the plasma samples. Of 87 proteins, 48 and 41 proteins were changed in the ageing and adult groups, respectively. Several distinctly expressed plasma proteins identified in CCDS were involved in complement and coagulation cascades and the apolipoprotein metabolism pathway. Plasma A?₄₂ levels considerably overlapped within the CCDS and ageing groups. In the adult group, the A?₄₂ level was low compared with that in the other groups. Nevertheless, plasma A?₄₂ did not show a correlation with the Canine Cognitive Dysfunction Rating scale (CCDR) score in the CCDS group (p =?0.131, R² =?0.261).

Conclusions

Our present findings suggest that plasma A?₄₂ does not show potential for use as a diagnostic biomarker in CCDS. The nano-LC-MS/MS data revealed that the predictive underlying mechanism of CCDS was the co-occurrence of inflammation-mediated acute phase response proteins and complement and coagulation cascades that partly functioned by apolipoproteins and lipid metabolism. Some of the differentially expressed proteins may serve as potential predictor biomarkers along with A?₄₂ in plasma for improved CCDS diagnosis. Further study in larger population-based cohort study is required in validation to define the correlation between protein expression and the pathogenesis of CCDS.

SUBMITTER: Phochantachinda S

PROVIDER: S-EPMC7845120 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature

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Publications

Change in the plasma proteome associated with canine cognitive dysfunction syndrome (CCDS) in Thailand.

Phochantachinda Sataporn S Chantong Boonrat B Reamtong Onrapak O Chatchaisak Duangthip D

BMC veterinary research 20210129 1

<h4>Background</h4>Canine cognitive dysfunction syndrome (CCDS) is a progressive neurodegenerative disorder found in senior dogs. Due to the lack of biological markers, CCDS is commonly underdiagnosed. The aim of this study was to identify potential plasma biomarkers using proteomics techniques and to increase our understanding of the pathogenic mechanism of the disease. Plasma amyloid beta 42 (Aβ<sub>42</sub>) has been seen to be a controversial biomarker for CCDS. Proteomics analysis was perfo ...[more]

PMID: 33514370

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Project description:Acute haemorrhagic diarrhoea is a common complaint in dogs. In addition to causes like intestinal parasites, dietary indiscretion, intestinal foreign bodies, canine parvovirus infection, or hypoadrenocorticism, acute haemorrhagic diarrhoea syndrome (AHDS) is an important and sometimes life-threatening differential diagnosis. There is some evidence supporting the link between Clostridium perfringens toxins and AHDS. These toxins may be partially responsible for the epithelial cell injury, but the pathogenesis of AHDS is still not fully understood. Recent studies have suggested that severe damage to the intestinal mucosa and associated barrier dysfunction can trigger chronic gastrointestinal illnesses. Besides bloodwork and classical markers for AHDS such as protein loss and intestinal bacterial dysbiosis, we focused mainly on the plasma-proteome to identify systemic pathological alterations during this disease and searched for potential biomarkers to improve the diagnosis. To accomplish the goals, we used liquid chromatography-mass spectrometry. We compared the proteomic profiles of 20 dogs with AHDS to 20 age-, breed-, and sex-matched control dogs. All dogs were examined, and several blood work parameters were determined and compared, including plasma biochemistry and cell counts. We identified and quantified (relative quantification) 207 plasmatic proteins, from which dozens showed significantly altered levels in AHDS. Serpina3, Lipopolysaccharide-binding protein, several Ig-like domain-containing proteins, Glyceraldehyde-3-phosphate dehydrogenase and Serum amyloid A were more abundant in plasma from AHDS affected dogs. In contrast, other proteins such as Paraoxonase, Selenoprotein, Amine oxidases, and Apolipoprotein C-IV were significantly less abundant. Many of the identified and quantified proteins are known to be associated with inflammation. Other proteins like Serpina3 and RPLP1 have a relevant role in oncogenesis. Some proteins and their roles have not yet been described in dogs with diarrhoea. Our study opens new avenues that could contribute to the understanding of the aetiology and pathophysiology of AHDS.

Dataset Information

Change in the plasma proteome associated with canine cognitive dysfunction syndrome (CCDS) in Thailand.

Background

Results

Conclusions

Publications

Change in the plasma proteome associated with canine cognitive dysfunction syndrome (CCDS) in Thailand.

Similar Datasets

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