Ontology highlight
ABSTRACT:
SUBMITTER: Donohue MJ
PROVIDER: S-EPMC7845746 | biostudies-literature | 2021 Jan
REPOSITORIES: biostudies-literature
Donohue Matthew J MJ Filla Robert T RT Steyer Daniel J DJ Eaton Wesley J WJ Roper Michael G MG
Journal of chromatography. A 20201215
Glucose homeostasis is maintained through the secretion of peptide hormones, such as insulin, somatostatin, and glucagon, from islets of Langerhans, clusters of endocrine cells found in the pancreas. This report describes an LC-MS method using multiple reaction monitoring for quantitation of insulin, C-peptide, glucagon, and somatostatin secretion from human islet populations. For rapid analysis, a 5 min separation was achieved using a 2.1 × 30 mm (i.d. x length) C18 column with 2.7 µm diameter ...[more]