A target enrichment probe set for resolving the flagellate land plant tree of life.

ABSTRACT:

Premise

New sequencing technologies facilitate the generation of large-scale molecular data sets for constructing the plant tree of life. We describe a new probe set for target enrichment sequencing to generate nuclear sequence data to build phylogenetic trees with any flagellate land plants, including hornworts, liverworts, mosses, lycophytes, ferns, and all gymnosperms.

Methods

We leveraged existing transcriptome and genome sequence data to design the GoFlag 451 probes, a set of 56,989 probes for target enrichment sequencing of 451 exons that are found in 248 single-copy or low-copy nuclear genes across flagellate plant lineages.

Results

Our results indicate that target enrichment using the GoFlag451 probe set can provide large nuclear data sets that can be used to

SUBMITTER: Breinholt JW 

PROVIDER: S-EPMC7845764 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature