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Extensive tissue-specific expression variation and novel regulators underlying circadian behavior.

ABSTRACT: Natural genetic variation affects circadian rhythms across the evolutionary tree, but the underlying molecular mechanisms are poorly understood. We investigated population-level, molecular circadian clock variation by generating >700 tissue-specific transcriptomes of Drosophila melanogaster (w¹¹¹⁸ ) and 141 Drosophila Genetic Reference Panel (DGRP) lines. This comprehensive circadian gene expression atlas contains >1700 cycling genes including previously unknown central circadian clock components and tissue-specific regulators. Furthermore, >30% of DGRP lines exhibited aberrant circadian gene expression, revealing abundant genetic variation-mediated, intertissue circadian expression desynchrony. Genetic analysis of one line with the strongest deviating circadian expression uncovered a novel cry mutation that, as shown by protein structural modeling and brain immunohistochemistry, disrupts the light-driven flavin adenine dinucleotide cofactor photoreduction, providing in vivo support for the importance of this conserved photoentrainment mechanism. Together, our study revealed pervasive tissue-specific circadian expression variation with genetic variants acting upon tissue-specific regulatory networks to generate local gene expression oscillations.

SUBMITTER: Litovchenko M

PROVIDER: S-EPMC7846174 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature

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Extensive tissue-specific expression variation and novel regulators underlying circadian behavior.

Litovchenko Maria M Meireles-Filho Antonio C A ACA Frochaux Michael V MV Bevers Roel P J RPJ Prunotto Alessio A Anduaga Ane Martin AM Hollis Brian B Gardeux Vincent V Braman Virginie S VS Russeil Julie M C JMC Kadener Sebastian S Dal Peraro Matteo M Deplancke Bart B

Science advances 20210129 5

Natural genetic variation affects circadian rhythms across the evolutionary tree, but the underlying molecular mechanisms are poorly understood. We investigated population-level, molecular circadian clock variation by generating >700 tissue-specific transcriptomes of Drosophila melanogaster (w1118 ) and 141 Drosophila Genetic Reference Panel (DGRP) lines. This comprehensive circadian gene expression atlas contains >1700 cycling genes including previously unknown c ...[more]

PMID: 33514540

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Extensive tissue-specific expression variation and novel regulators underlying circadian behavior.

Publications

Extensive tissue-specific expression variation and novel regulators underlying circadian behavior.

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