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Isolation and characterization of a novel hydrocarbonoclastic and biosurfactant producing bacterial strain: Fictibacillus phosphorivorans RP3.

ABSTRACT: In this study, an indigenous novel hydrocarbonoclastic (kerosene and diesel degrading) and biosurfactant producing strain Fictibacillus phosphorivorans RP3 was identified. The characteristics of bacterial strain were ascertained through its unique morphological and biochemical attributes, 16S rRNA sequencing, and phylogenetic analysis. The degradation of hydrocarbons by F. phosphorivorans RP3 was observed at Day 7, Day 10 and Day 14 of the experimental duration. GC-FID chromatograms demonstrated a significant increase in hydrocarbon degradation (%) with progressing days (from 7 to 14). The bacterium exhibited capability to utilize and degrade n-hexadecane (used for primary screening) and petroleum hydrocarbons (kerosene and diesel; by ≥ 90%). With increase in the number of experimentation days, the optical density of the culture medium increased, whereas pH declined (became acidic) for both Kerosene and Diesel. Absence of resistance to routinely used antibiotics makes it an ideal candidate for future field application. The study is, thus, significant in view of toxicological implications of hydrocarbons and their degradation using environmentally safe techniques so as to maintain ecological and human health.

SUBMITTER: Pandey R

PROVIDER: S-EPMC7847478 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature

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Isolation and characterization of a novel hydrocarbonoclastic and biosurfactant producing bacterial strain: Fictibacillus phosphorivorans RP3.

Pandey Ranjan R Sharma Padma P Rathee Sonia S Singh Harminder Pal HP Batish Daizy Rani DR Krishnamurthy Bhaskar B Kohli Ravinder Kumar RK

3 Biotech 20210130 2

In this study, an indigenous novel hydrocarbonoclastic (kerosene and diesel degrading) and biosurfactant producing strain Fictibacillus phosphorivorans RP3 was identified. The characteristics of bacterial strain were ascertained through its unique morphological and biochemical attributes, 16S rRNA sequencing, and phylogenetic analysis. The degradation of hydrocarbons by F. phosphorivorans RP3 was observed at Day 7, Day 10 and Day 14 of the experimental duration. GC-FID chrom ...[more]

PMID: 33552833

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Project description:BackgroundChitin is an important biopolymer next to cellulose, extracted in the present study. The exoskeleton of marine bycatch brachyuran crabs, namely Calappa lophos, Dromia dehaani, Dorippe facchino and also from stomatopod Squilla spp. were used to extract chitin through fermentation methods by employing two bacterial strains such as Pseudomonas aeruginosa, Serratia marcescens. The yield of chitin was 44.24%, 37.45%, 11.56% and 27.24% in C. lophos, D. dehaani, D. facchino and Squilla spp. respectively. FT-IR spectra of the produced chitin exhibit peaks which is more or less coherent to that of standard chitin which is further analysed by Scanning Electron Microscope. The quality of produced chitin was assessed through moisture, protein, ash and lipid content analysis ensured that chitin obtained from trash crustaceans are on par with that of standard chitin.ResultsA total of 10 samples were collected from different areas of Jiangsu China for screening of chitinase-producing bacteria. Based on the clearance zone, two of the best samples were chosen for further study. 16S rRNA sequence analysis showed that this strain belongs to genus Myxococcus and species Myxococcus fulvus. Phylogenetic analysis was performed and it shows strain UM01 is a novel bacterial strain. UM01 isolate shows maximum chitinase production at 35 °C and 8 pH. Among all, these colloidal chitins were found to be the best for chitinase production. Three chitinase-producing genes were identified and sequenced by using degenerative plasmid. UMCda gene (chitin disaccharide deacetylase) was cloned into E. coli DH5a by using PET-28a vector, and antagonistic activity was examined against T. reesei.ConclusionTo our knowledge, this is the earliest study report to gene cloning and identification of the chitinase gene in Myxococcus fulvus. Chitinase plays a key role in decomposition and utilization of chitin as a raw material. This research indicates that Myxococcus fulvus UM01 strain is a novel myxobacteria strain and can produce large amounts of chitinase within a short time. The UMCda gene cloned into E. coli DH5a showed a promising effect as antifungal activity. In overall findings, the specific strain UM01 has endowed properties of bioconversation of waste chitin and other biological applications.

Project description:Microbial biosurfactants, produced by fungi, yeast, and bacteria, are surface-active compounds with emulsifying properties that have a number of known activities, including the solubilization of microbial biofilms. In an on-going survey to uncover new or enhanced antimicrobial metabolite-producing microbes from harsh environments, such as oil-rich niches, 123 bacterial strains were isolated from three oil batteries in the region of Chauvin, Alberta, and characterized by 16S rRNA gene sequencing. Based on their nucleotide sequences, the strains are associated with 3 phyla (Actinobacteria, Proteobacteria and Firmicutes), as well as 17 other discrete genera that shared high homology with known sequences, with the majority of these strains identified to the species level. The most prevalent strains associated with the three oil wells belonged to the Bacillus genus. Thirty-four of the 123 strains were identified as biosurfactant-producers, among which Bacillus methylotrophicus strain OB9 exhibited the highest biosurfactant activity based on multiple screening methods and a comparative analysis with the commercially available biosurfactant, Tween 20. B. methylotrophicus OB9 was selected for further antimicrobial analysis and addition of live cultures of B. methylotrophicus OB9 (or partially purified biosurfactant fractions thereof) were highly effective on biofilm disruption in agar diffusion assays against several Gram-negative food-borne bacteria and plant pathogens. Upon co-culturing with B. methylotrophicus OB9, the number of either Salmonella enterica subsp. enterica Newport SL1 or Xanthomonas campestris B07.007 cells significantly decreased after 6 h and were not retrieved from co-cultures following 12 h exposure. These results also translated to studies on plants, where bacterized tomato seedlings with OB9 significantly protected the tomato leaves from Salmonella enterica Newport SL1 contamination, as evidenced by a 40% reduction of log10 CFU of Salmonella/mg leaf tissue compared to non-bacterized tomato leaves. When B. methylotrophicus 0B9 was used for bacterized lettuce, the growth of X. campestris B07.007, the causal agent of bacterial leaf spot of lettuce, was completely inhibited. While limited, these studies are noteworthy as they demonstrate the inhibition spectrum of B. methylotrophicus 0B9 against both human and plant pathogens; thereby making this bacterium attractive for agricultural and food safety applications in a climate where microbial-biofilm persistence is an increasing problem.

Dataset Information

Isolation and characterization of a novel hydrocarbonoclastic and biosurfactant producing bacterial strain: Fictibacillus phosphorivorans RP3.

Publications

Isolation and characterization of a novel hydrocarbonoclastic and biosurfactant producing bacterial strain: <i>Fictibacillus phosphorivorans</i> RP3.

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