Dataset Information
Characterization of the intestinal mucosal proteome in cats with inflammatory bowel disease and alimentary small cell lymphoma.
Ontology highlight
ABSTRACT: Background
Current tests for diagnosis and differentiation of lymphoplasmacytic enteritis (LPE) and small cell lymphoma (SCL) in cats are expensive, invasive, and lack specificity. The identification of less invasive, more reliable biomarkers would facilitate diagnosis.Objectives
To characterize the mucosal proteome in endoscopically obtained, small intestinal tissue biopsy specimens. We hypothesized that differentially expressed proteins could be identified and serve as biomarker candidates for the differentiation of LPE and SCL in cats.Animals
Six healthy control cats, 6 cats with LPE, and 8 cats with SCL.Methods
The mucosal proteome was analyzed using 2-dimensional fluorescence difference gel electrophoresis (2D DIGE) and nanoflow liquid chromatography tandem mass spectrometry. For 5 proteins, results were verified by Western blot analysis.Results
A total of 2349 spots were identified, of which 9 were differentially expressed with a ?2-fold change between healthy cats and cats with LPE and SCL (.01?< P Conclusions and clinical importanceTwo-D DIGE did not identify potential biomarker candidates in the intestinal mucosa of cats with LPE and SCL. Future studies should focus on different techniques to identify biomarker candidates for cats with chronic enteropathies (CE).
SUBMITTER: Marsilio S
PROVIDER: S-EPMC7848303 | biostudies-literature | 2021 Jan
REPOSITORIES: biostudies-literature
Publications
Characterization of the intestinal mucosal proteome in cats with inflammatory bowel disease and alimentary small cell lymphoma.
Journal of veterinary internal medicine 20210120 1
<h4>Background</h4>Current tests for diagnosis and differentiation of lymphoplasmacytic enteritis (LPE) and small cell lymphoma (SCL) in cats are expensive, invasive, and lack specificity. The identification of less invasive, more reliable biomarkers would facilitate diagnosis.<h4>Objectives</h4>To characterize the mucosal proteome in endoscopically obtained, small intestinal tissue biopsy specimens. We hypothesized that differentially expressed proteins could be identified and serve as biomarke ...[more]
