Human-specific GAPDH qRT-PCR is an accurate and sensitive method of xenograft metastasis quantification.

ABSTRACT: Metastasis is the primary cause of cancer-related mortality. Experimental models that accurately reflect changes in metastatic burden are essential tools for developing treatments and to gain a better understanding of disease. Murine xenograft tumor models mimic the human scenario and provide a platform for metastasis analyses. An ex vivo quantitative method, gaining favor for its ease and accuracy, is quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR); however, it is currently unclear how well this method correlates with gold-standard histological analysis, and its use has required detection of overexpressed exogenous genes. We have introduced a variation of the qRT-PCR method: human-specific glyceraldehyde 3-phosphate dehydrogenase (GAPDH) qRT-PCR, which allows

SUBMITTER: Dahn ML 

PROVIDER: S-EPMC7848707 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature