Unknown

Dataset Information

0

Silenced lncRNA H19 and up-regulated microRNA-129 accelerates viability and restrains apoptosis of PC12 cells induced by Aβ25-35 in a cellular model of Alzheimer's disease.

ABSTRACT: Accumulating data manifest that long non-coding RNA (lncRNAs) are involved in all kinds of neurodegenerative disorders, consisting of the onset and progression of Alzheimer's disease (AD). The study was for the research of the mechanism of lncRNA H19 (H19) in viability and apoptosis of PC12 cells induced by Aβ25-35 in a cellular model of AD with the regulation of microRNA (miR)-129 and high mobility group box-1 protein (HMGB1). An AD cellular model of PC12 cells was established using Aβ25-35. The Aβ25-35-induced PC12 cells were transfected with si-H19 or miR-129 mimic to figure their roles in cell viability,apoptosis, mitochondrial membrane potential dysfunction and oxidative stress in AD. Luciferase reporter assay and RNA-pull down assay were employed for verification of the binding relationship between H19 and miR-129 and the targeting relationship between miR-129 and HMGB1. An AD mouse model was induced and brain tissues were collected. H19, miR-129 and HMGB1 were detected in Aβ25-35-treated cells and brain tissues of AD mice. Elevated H19, HMGB1 and decreased miR-129 were found in Aβ25-35-treated PC12 cells as well as in brain tissues of AD mice. Silenced H19 or elevated miR-129 promoted viability, inhibited apoptosis, prevented mitochondrial membrane potential dysfunction and decreased oxidative stress in Aβ25-35-treated PC12 cells. H19 could specifically bind to miR-129. MiR-129 specifically suppressed HMGB1 expression. This study suggests that silenced H19 and up-regulated miR-129 accelerates viability and represses apoptosis of PC12 cells stimulated by Aβ25-35 in AD, which is beneficial for AD treatment.

SUBMITTER: Zhang YY 

PROVIDER: S-EPMC7849745 | biostudies-literature |

REPOSITORIES: biostudies-literature

Json Xml

Similar Datasets

Unknown Mulberry extracts alleviate aβ 25-35-induced injury and change the gene expression profile in PC12 cells.
| S-EPMC4280656 | biostudies-literature
Unknown <i>Sideritis scardica</i> Extracts Demonstrate Neuroprotective Activity against Aβ<sub>25-35</sub> Toxicity.
| S-EPMC10142657 | biostudies-literature
Unknown Dendrobium alkaloids prevent Aβ<sub>25-35</sub>-induced neuronal and synaptic loss via promoting neurotrophic factors expression in mice.
| S-EPMC5157189 | biostudies-literature
Unknown lncRNA and circRNA expression profiles in the hippocampus of Aβ<sub>25‑35</sub>‑induced AD mice treated with Tripterygium glycoside.
| S-EPMC10433443 | biostudies-literature
Unknown Trilobatin Protects Against Aβ<sub>25-35</sub>-Induced Hippocampal HT22 Cells Apoptosis Through Mediating ROS/p38/Caspase 3-Dependent Pathway.
| S-EPMC7248209 | biostudies-literature
Unknown MGCD0103, a selective histone deacetylase inhibitor, coameliorates oligomeric Aβ<sub>25-35</sub> -induced anxiety and cognitive deficits in a mouse model.
| S-EPMC6488906 | biostudies-literature
Unknown lncRNA-Associated Competitive Endogenous RNA Regulatory Network in an Aβ<sub>25-35</sub>-Induced AD Mouse Model Treated with Tripterygium Glycoside.
| S-EPMC8141406 | biostudies-literature
Unknown Dendrobium nobile Lindl alkaloid, a novel autophagy inducer, protects against axonal degeneration induced by Aβ<sub>25-35</sub> in hippocampus neurons in vitro.
| S-EPMC6492701 | biostudies-literature
Unknown Panaxcerol D from <i>Panax ginseng</i> ameliorates the memory impairment induced by cholinergic blockade or Aβ<sub>25-35</sub> peptide in mice.
| S-EPMC10785420 | biostudies-literature
Unknown Component of Cannabis, Cannabidiol, as a Possible Drug against the Cytotoxicity of Aβ(31-35) and Aβ(25-35) Peptides: An Investigation by Molecular Dynamics and Well-Tempered Metadynamics Simulations.
| S-EPMC8023578 | biostudies-literature