Ontology highlight
ABSTRACT:
SUBMITTER: Hamilton JR
PROVIDER: S-EPMC7852249 | biostudies-literature | 2021 Jan
REPOSITORIES: biostudies-literature
Hamilton Jennifer R JR Stahl Elizabeth C EC Tsuchida Connor A CA Lin-Shiao Enrique E Tsui C Kimberly CK Pestal Kathleen K Gildea Holly K HK Witkowsky Lea B LB Moehle Erica A EA McDevitt Shana L SL McElroy Matthew M Keller Amanda A Sylvain Iman I Hirsh Ariana A Ciling Alison A Ehrenberg Alexander J AJ Ringeisen Bradley R BR Huberty Garth G Urnov Fyodor D FD Giannikopoulos Petros P Doudna Jennifer A JA
medRxiv : the preprint server for health sciences 20210129
Saliva is an attractive specimen type for asymptomatic surveillance of COVID-19 in large populations due to its ease of collection and its demonstrated utility for detecting RNA from SARS-CoV-2. Multiple saliva-based viral detection protocols use a direct-to-RT-qPCR approach that eliminates nucleic acid extraction but can reduce viral RNA detection sensitivity. To improve test sensitivity while maintaining speed, we developed a robotic nucleic acid extraction method for detecting SARS-CoV-2 RNA ...[more]