Flow-through stable isotope probing (Flow-SIP) minimizes cross-feeding in complex microbial communities.
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ABSTRACT:
SUBMITTER: Mooshammer M
PROVIDER: S-EPMC7852690 | biostudies-literature | 2021 Jan
REPOSITORIES: biostudies-literature
Publications
Flow-through stable isotope probing (Flow-SIP) minimizes cross-feeding in complex microbial communities.
Mooshammer Maria M Kitzinger Katharina K Schintlmeister Arno A Ahmerkamp Soeren S Nielsen Jeppe Lund JL Nielsen Per Halkjær PH Wagner Michael M
The ISME journal 20200902 1
Stable isotope probing (SIP) is a key tool for identifying the microorganisms catalyzing the turnover of specific substrates in the environment and to quantify their relative contributions to biogeochemical processes. However, SIP-based studies are subject to the uncertainties posed by cross-feeding, where microorganisms release isotopically labeled products, which are then used by other microorganisms, instead of incorporating the added tracer directly. Here, we introduce a SIP approach that ha ...[more]
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