Dataset Information

Temperature response of wheat affects final height and the timing of stem elongation under field conditions.

ABSTRACT: In wheat, temperature affects the timing and intensity of stem elongation (SE). Genetic variation for this process is therefore important for adaptation. This study investigates the genetic response to temperature fluctuations during SE and its relationship to phenology and height. Canopy height of 315 wheat genotypes (GABI wheat panel) was scanned twice weekly in the field phenotyping platform (FIP) of ETH Zurich using a LIDAR. Temperature response was modelled using linear regressions between SE and mean temperature in each measurement interval. This led to a temperature-responsive (slope) and a temperature-irresponsive (intercept) component. The temperature response was highly heritable (H2 = 0.81) and positively related to a later start and end of SE as well as final height. Genome-wide association mapping revealed three temperature-responsive and four temperature-irresponsive quantitative trait loci (QTL). Furthermore, putative candidate genes for temperature-response QTL were frequently related to the flowering pathway in A. thaliana, whereas temperature-irresponsive QTLs corresponded with growth and reduced height genes. In combination with Rht and Ppd alleles, these loci, together with the loci for the timing of SE accounted for 71% of the variability in height. This demonstrates how high-throughput field phenotyping combined with environmental covariates can contribute to a smarter selection of climate-resilient crops.

SUBMITTER: Kronenberg L

PROVIDER: S-EPMC7853599 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature

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Temperature response of wheat affects final height and the timing of stem elongation under field conditions.

Kronenberg Lukas L Yates Steven S Boer Martin P MP Kirchgessner Norbert N Walter Achim A Hund Andreas A

Journal of experimental botany 20210201 2

In wheat, temperature affects the timing and intensity of stem elongation. Genetic variation for this process is therefore important for adaptation. This study investigates the genetic response to temperature fluctuations during stem elongation and its relationship to phenology and height. Canopy height of 315 wheat genotypes (GABI wheat panel) was scanned twice weekly in the field phenotyping platform (FIP) of ETH Zurich using a LIDAR. Temperature response was modelled using linear regressions ...[more]

PMID: 33057698

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Project description:Cell proliferation is achieved by numerous enzyme reactions. Temperature governs the activity of each enzyme, which overall determines the optimal growth temperature. Synthesizing useful chemicals and fuels utilizes only part of the metabolic pathways, especially the central metabolic pathways such as glycolysis and TCA cycle to metabolize glucose. However, the optimal temperature for the activity of the central metabolic pathways is inconclusive whether it is correlated with the optimal temperature for cell proliferation. Here, we found that Corynebacterium glutamicum wild type increased the metabolic activity to consume glucose under anaerobic (oxygen deprived) conditions at 42.5ºC, in which the cell hardly grows under aerobic conditions. Glucose consumption rate was increased by 24% at 42.5ºC compared to that at the optimal growth temperature of 30.0ºC. Transcriptional analysis showed that gapX gene encoding glycelaldehydre-3-phosphate dehydrogenase, glucokinase gene, and a gene involved in glucose uptake were upregulated at 42.5ºC. Production of fermentative lactate was increased by 69% than that at 30.0ºC, whereas succinate production was decreased by 13%. In addition to several glycolytic enzymes, the activity of pyruvate kinase was increased with increasing the temperature, whereas the activity of phosphoenolpyruvate carboxylase in anapleotic pathway leading to succinate synthesis was decreased. However, a metabolically engineered succinate over-producing strain, in which lactate production was shut off and pyruvate carboxylase encoding gene in another anapleotic pathway was overexpressed, produced 34% higher succinate at 42.5ºC than that at 30.0ºC with increased glucose consumption. This study provides the evidence that the optimal reaction temperature for production of fermentative products can be set beyond upper limit of growth temperature in C. glutamicum, and hence it could be applicable for producing various chemicals and fuels in C. glutamicum under anaerobic conditions and non-growing cell reactions in other microorganisms.

Dataset Information

Temperature response of wheat affects final height and the timing of stem elongation under field conditions.

Publications

Temperature response of wheat affects final height and the timing of stem elongation under field conditions.

Similar Datasets

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