Ontology highlight
ABSTRACT:
SUBMITTER: Mikolajewicz N
PROVIDER: S-EPMC7854243 | biostudies-literature | 2019 May
REPOSITORIES: biostudies-literature
Mikolajewicz Nicholas N Komarova Svetlana V SV
Bio-protocol 20190520 10
Extracellular ATP is a potent signaling molecule that stimulates intracellular calcium responses through purinergic (P2) receptors in mammalian cells. While extracellular ATP and intracellular calcium can be measured separately, simultaneous monitoring can offer additional insights into P2 receptor physiology. This protocol takes advantage of the overlapping fluorescence spectra between the ATP-detection substrate luciferin and calcium indicator dye Fura2. Mammalian cells are loaded with Fura2-A ...[more]