Ontology highlight
ABSTRACT:
SUBMITTER: Walsh AJ
PROVIDER: S-EPMC7854821 | biostudies-literature | 2021 Jan
REPOSITORIES: biostudies-literature
Nature biomedical engineering 20200727 1
The function of a T cell depends on its subtype and activation state. Here, we show that imaging of the autofluorescence lifetime signals of quiescent and activated T cells can be used to classify the cells. T cells isolated from human peripheral blood and activated in culture using tetrameric antibodies against the surface ligands CD2, CD3 and CD28 showed specific activation-state-dependent patterns of autofluorescence lifetime. Logistic regression models and random forest models classified T c ...[more]