Dataset Information

Interacting Abiotic Factors Affect Growth and Aflatoxin B₁ Production Profiles of Aspergillus flavus Strains on Pistachio-Based Matrices and Pistachio Nuts.

ABSTRACT: Pistachio nuts are an economically important commodity produced by many countries. They can be colonized by mycotoxigenic fungi, especially Aspergillus flavus, resulting in contamination with aflatoxins (AFs), especially aflatoxin B₁ (AFB₁), a Class 1a carcinogen. The objectives were to examine the effect of interactions between the two key abiotic factors, temperature and water activity (a _w ) on (a) in vitro growth and AFB₁ production by four strains of A. flavus isolated from pistachio nuts, on a milled pistachio nut medium modified ionically (NaCl) and non-ionically (glycerol) in the range 20-35°C and 0.995-0.85 a _w , (b) colonization of layers of raw pistachio nuts stored at different interacting temperature x a _w conditions and on relative AFB₁ production and (c) develop models to produce contour maps of the optimal and marginal boundary conditions for growth and AFB₁ production by up to 4 strains of this species. On pistachio nut-based media, optimum growth of four strains of A. flavus was at 0.98-0.95 a _w and 30-35°C. Optimum AFB₁ production was at 30-35°C and 0.98 a _w . No significant differences in growth was found on ionic and non-ionically modified media. Colonization of layers of raw pistachio nuts was slower and contamination with AFB₁ significantly less than in in vitro studies. Contour maps based on the pooled data for up to four strains (in vitro, in situ) showed the optimum and marginal conditions for growth and AFB₁ production. These data can be used to identify those conditions which represent a high, intermediate or low risk of colonization and AFB₁ contamination in the pistachio nut processing chain. These results are discussed in the context of the development of appropriate intervention strategies to minimize AFB₁ contamination of this economically important commodity.

SUBMITTER: Baazeem A

PROVIDER: S-EPMC7855869 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Publications

Interacting Abiotic Factors Affect Growth and Aflatoxin B1 Production Profiles of Aspergillus flavus Strains on Pistachio-Based Matrices and Pistachio Nuts.

Baazeem Alaa A Garcia-Cela Esther E Medina Angel A Magan Naresh N

Frontiers in microbiology 20210120

Pistachio nuts are an economically important commodity produced by many countries. They can be colonized by mycotoxigenic fungi, especially Aspergillus flavus, resulting in contamination with aflatoxins (AFs), especially aflatoxin B1 (AFB1), a Class 1a carcinogen. The objectives were to examine the effect of interactions between the two key abiotic factors, temperature and water activity (a w ) on (a) in vitro growth and AFB1</sub ...[more]

PMID: 33552034

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Project description:Pistachio nuts are an important economic tree nut crop which is used directly or processed for many food-related activities. They can become colonized by mycotoxigenic spoilage fungi, especially Aspergillus flavus, mainly resulting in contamination with aflatoxins (AFs), especially aflatoxin B1 (AFB1). The prevailing climate in which these crops are grown changes as temperature and atmospheric CO2 levels increase, and episodes of extreme wet/dry cycles occur due to human industrial activity. The objectives of this study were to evaluate the effect of interacting Climate Change (CC)-related abiotic factors of temperature (35 vs. 37 °C), CO2 (400 vs. 1000 ppm), and water stress (0.98-0.93 water activity, aw) on (a) growth (b) aflD and aflR biosynthetic gene expression and (c) AFB1 production by two strains A. flavus (AB3, AB10) in vitro on milled pistachio-based media and when colonizing layers of shelled raw pistachio nuts. The A. flavus strains were resilient in terms of growth on pistachio-based media and the colonisation of pistachio nuts with no significant difference when exposed to the interacting three-way climate-related abiotic factors. However, in vitro studies showed that AFB1 production was significantly stimulated (p < 0.05), especially when exposed to 1000 ppm CO2 at 0.98-0.95 aw and 35 °C, and sometimes in the 37 °C treatment group at 0.98 aw. The relative expression of the structural aflD gene involved in AFB1 biosynthesis was decreased or only slightly increased, relative to the control conditions at elevated CO, regardless of the aw level examined. For the regulatory aflR gene expression, there was a significant (p < 0.05) increase in 1000 ppm CO2 and 37 °C for both strains, especially at 0.95 aw. The in situ colonization of pistachio nuts resulted in a significant (p < 0.05) stimulation of AFB1 production at 35 °C and 1000 ppm CO2 for both strains, especially at 0.98 aw. At 37 °C, AFB1 production was either decreased, in strain AB3, or remained similar, as in strain AB10, when exposed to 1000 ppm CO2. This suggests that CC factors may have a differential effect, depending on the interacting conditions of temperature, exposure to CO2 and the level of water stress on AFB1 production.

Dataset Information

Interacting Abiotic Factors Affect Growth and Aflatoxin B₁ Production Profiles of Aspergillus flavus Strains on Pistachio-Based Matrices and Pistachio Nuts.

Publications

Interacting Abiotic Factors Affect Growth and Aflatoxin B<sub>1</sub> Production Profiles of <i>Aspergillus flavus</i> Strains on Pistachio-Based Matrices and Pistachio Nuts.

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