Ontology highlight
ABSTRACT:
SUBMITTER: Becker KA
PROVIDER: S-EPMC7857112 | biostudies-literature | 2021 Mar
REPOSITORIES: biostudies-literature
Becker Katrin Anne KA Carpinteiro Alexander A Hoffmann Markus M Pöhlmann Stefan S Kornhuber Johannes J Gulbins Erich E
STAR protocols 20210203 1
This protocol enables the testing of drugs against infection of epithelial cells with SARS-CoV-2 (severe acute respiratory syndrome coronavirus-2), using pseudo-typed replication deficient vesicular stomatitis virus particles (pp-VSV) presenting the SARS-CoV-2 spike protein. After treating human volunteers with amitriptyline, an approved antidepressant and inhibitor of the acid sphingomyelinase, freshly isolated nasal epithelial cells were infected <i>ex vivo</i> and infection levels were quanti ...[more]