Dataset Information

OCLSTM: Optimized convolutional and long short-term memory neural network model for protein secondary structure prediction.

ABSTRACT: Protein secondary structure prediction is extremely important for determining the spatial structure and function of proteins. In this paper, we apply an optimized convolutional neural network and long short-term memory neural network models to protein secondary structure prediction, which is called OCLSTM. We use an optimized convolutional neural network to extract local features between amino acid residues. Then use the bidirectional long short-term memory neural network to extract the remote interactions between the internal residues of the protein sequence to predict the protein structure. Experiments are performed on CASP10, CASP11, CASP12, CB513, and 25PDB datasets, and the good performance of 84.68%, 82.36%, 82.91%, 84.21% and 85.08% is achieved respectively. Experimental results show that the model can achieve better results.

SUBMITTER: Zhao Y

PROVIDER: S-EPMC7857624 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature

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OCLSTM: Optimized convolutional and long short-term memory neural network model for protein secondary structure prediction.

Zhao Yawu Y Liu Yihui Y

PloS one 20210203 2

Protein secondary structure prediction is extremely important for determining the spatial structure and function of proteins. In this paper, we apply an optimized convolutional neural network and long short-term memory neural network models to protein secondary structure prediction, which is called OCLSTM. We use an optimized convolutional neural network to extract local features between amino acid residues. Then use the bidirectional long short-term memory neural network to extract the remote i ...[more]

PMID: 33534819

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Project description:Experimental techniques for measuring chromatin accessibility are expensive and time consuming, appealing for the development of computational approaches to predict open chromatin regions from DNA sequences. Along this direction, existing methods fall into two classes: one based on handcrafted k -mer features and the other based on convolutional neural networks. Although both categories have shown good performance in specific applications thus far, there still lacks a comprehensive framework to integrate useful k -mer co-occurrence information with recent advances in deep learning.We fill this gap by addressing the problem of chromatin accessibility prediction with a convolutional Long Short-Term Memory (LSTM) network with k -mer embedding. We first split DNA sequences into k -mers and pre-train k -mer embedding vectors based on the co-occurrence matrix of k -mers by using an unsupervised representation learning approach. We then construct a supervised deep learning architecture comprised of an embedding layer, three convolutional layers and a Bidirectional LSTM (BLSTM) layer for feature learning and classification. We demonstrate that our method gains high-quality fixed-length features from variable-length sequences and consistently outperforms baseline methods. We show that k -mer embedding can effectively enhance model performance by exploring different embedding strategies. We also prove the efficacy of both the convolution and the BLSTM layers by comparing two variations of the network architecture. We confirm the robustness of our model to hyper-parameters by performing sensitivity analysis. We hope our method can eventually reinforce our understanding of employing deep learning in genomic studies and shed light on research regarding mechanisms of chromatin accessibility.The source code can be downloaded from https://github.com/minxueric/ismb2017_lstm .tingchen@tsinghua.edu.cn or ruijiang@tsinghua.edu.cn.Supplementary materials are available at Bioinformatics online.

Project description:BackgroundThe accurate temporal analysis of muscle activation is of great interest in many research areas, spanning from neurorobotic systems to the assessment of altered locomotion patterns in orthopedic and neurological patients and the monitoring of their motor rehabilitation. The performance of the existing muscle activity detectors is strongly affected by both the SNR of the surface electromyography (sEMG) signals and the set of features used to detect the activation intervals. This work aims at introducing and validating a powerful approach to detect muscle activation intervals from sEMG signals, based on long short-term memory (LSTM) recurrent neural networks.MethodsFirst, the applicability of the proposed LSTM-based muscle activity detector (LSTM-MAD) is studied through simulated sEMG signals, comparing the LSTM-MAD performance against other two widely used approaches, i.e., the standard approach based on Teager-Kaiser Energy Operator (TKEO) and the traditional approach, used in clinical gait analysis, based on a double-threshold statistical detector (Stat). Second, the effect of the Signal-to-Noise Ratio (SNR) on the performance of the LSTM-MAD is assessed considering simulated signals with nine different SNR values. Finally, the newly introduced approach is validated on real sEMG signals, acquired during both physiological and pathological gait. Electromyography recordings from a total of 20 subjects (8 healthy individuals, 6 orthopedic patients, and 6 neurological patients) were included in the analysis.ResultsThe proposed algorithm overcomes the main limitations of the other tested approaches and it works directly on sEMG signals, without the need for background-noise and SNR estimation (as in Stat). Results demonstrate that LSTM-MAD outperforms the other approaches, revealing higher values of F1-score (F1-score > 0.91) and Jaccard similarity index (Jaccard > 0.85), and lower values of onset/offset bias (average absolute bias < 6 ms), both on simulated and real sEMG signals. Moreover, the advantages of using the LSTM-MAD algorithm are particularly evident for signals featuring a low to medium SNR.ConclusionsThe presented approach LSTM-MAD revealed excellent performances against TKEO and Stat. The validation carried out both on simulated and real signals, considering normal as well as pathological motor function during locomotion, demonstrated that it can be considered a powerful tool in the accurate and effective recognition/distinction of muscle activity from background noise in sEMG signals.

Dataset Information

OCLSTM: Optimized convolutional and long short-term memory neural network model for protein secondary structure prediction.

Publications

OCLSTM: Optimized convolutional and long short-term memory neural network model for protein secondary structure prediction.

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