Ontology highlight
ABSTRACT:
SUBMITTER: Wang Y
PROVIDER: S-EPMC7860141 | biostudies-literature | 2021 Jan
REPOSITORIES: biostudies-literature
Wang Yuxi Y Zhang Yong Y Chen Junbo J Wang Minjin M Zhang Ting T Luo Wenxin W Li Yalun Y Wu Yangping Y Zeng Bo B Zhang Kaixiang K Deng Ruijie R Li Weimin W
Analytical chemistry 20210129 7
The outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) caused a global health emergency, and its gene mutation and evolution further posed uncertainty of epidemic risk. Herein, we reported a light-up CRISPR-Cas13 transcription amplification method, which enables the detection of SARS-CoV-2 and its mutated variants. Sequence specificity was ensured by both the ligation process and Cas13a/crRNA recognition, allowing us to identify viral RNA mutation. Light-up RNA aptamer allo ...[more]