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FeII 4L4 Tetrahedron-Assisted Three-Way Junction Probe for Multiple miRNA Detection.


ABSTRACT: MicroRNAs (miRNAs) modulate a variety of cellular signaling pathways and play a vital role in cell-to-cell communication. The overlapped expression of a certain miRNA is commonly reported to be related to cancers. Therefore, combined detection of multiple miRNAs is of great significance for cancer diagnosis. Herein, we developed a FeII 4L4 tetrahedron-assisted three-way junction (3WJ) probe, which exhibited a higher stability than the normal 3WJ probe, for multiple miRNA detection. In this method, the simultaneous existence of miRNA-21 and miRNA-144 triggers the release of the Y3 sequence in the FeII 4L4 tetrahedron-assisted 3WJ probe, which in turn triggers subsequent CRISPR-Cas12a-assisted rolling circle amplification. Based on this, simultaneous detection of miRNA-21 and miRNA-144 was achieved. Furthermore, we also applied this method to the detection of miRNAs in clinical samples and achieved good agreement with quantitative real-time polymerase chain reaction (qRT-PCR), indicating its significant potentials in early diagnosis and treatment of cancer.

SUBMITTER: Qu CY 

PROVIDER: S-EPMC7860230 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature

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Fe<sup>II</sup> <sub>4</sub>L<sub>4</sub> Tetrahedron-Assisted Three-Way Junction Probe for Multiple miRNA Detection.

Qu Chun-Yan CY   Zhou Li-Sha LS   Shu Li-Hong LH   Huang Qian Q  

ACS omega 20210120 4


MicroRNAs (miRNAs) modulate a variety of cellular signaling pathways and play a vital role in cell-to-cell communication. The overlapped expression of a certain miRNA is commonly reported to be related to cancers. Therefore, combined detection of multiple miRNAs is of great significance for cancer diagnosis. Herein, we developed a Fe<sup>II</sup> <sub>4</sub>L<sub>4</sub> tetrahedron-assisted three-way junction (3WJ) probe, which exhibited a higher stability than the normal 3WJ probe, for multip  ...[more]

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