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PASA: Proteomic analysis of serum antibodies web server.

ABSTRACT:

Motivation

A comprehensive characterization of the humoral response towards a specific antigen requires quantification of the B-cell receptor repertoire by next-generation sequencing (BCR-Seq), as well as the analysis of serum antibodies against this antigen, using proteomics. The proteomic analysis is challenging since it necessitates the mapping of antigen-specific peptides to individual B-cell clones.

Results

The PASA web server provides a robust computational platform for the analysis and integration of data obtained from proteomics of serum antibodies. PASA maps peptides derived from antibodies raised against a specific antigen to corresponding antibody sequences. It then analyzes and integrates proteomics and BCR-Seq data, thus providing a comprehensive characterization of the humoral response. The PASA web server is freely available at https://pasa.tau.ac.il and open to all users without a login requirement.

SUBMITTER: Avram O

PROVIDER: S-EPMC7861515 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature

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PASA: Proteomic analysis of serum antibodies web server.

Avram Oren O Kigel Aya A Vaisman-Mentesh Anna A Kligsberg Sharon S Rosenstein Shai S Dror Yael Y Pupko Tal T Wine Yariv Y

PLoS computational biology 20210125 1

<h4>Motivation</h4>A comprehensive characterization of the humoral response towards a specific antigen requires quantification of the B-cell receptor repertoire by next-generation sequencing (BCR-Seq), as well as the analysis of serum antibodies against this antigen, using proteomics. The proteomic analysis is challenging since it necessitates the mapping of antigen-specific peptides to individual B-cell clones.<h4>Results</h4>The PASA web server provides a robust computational platform for the ...[more]

PMID: 33493161

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