Dataset Information

Acute exercise impacts AhR and PD-1 levels of CD8⁺ T-cells-Exploratory results from a randomized cross-over trial comparing endurance versus resistance exercise.

ABSTRACT:

Purpose

The programmed cell death protein 1 (PD-1) has become a promising target in cancer immunotherapy. PD-1 expression of CD8⁺ T-cells may be increased via the exploitation of aryl hydrocarbon receptor (AhR) signaling with kynurenine (KYN) as a ligand. Since exercise affects KYN metabolism, we exploratory investigated the influence of acute exercise bouts on AhR and PD-1 levels of CD8⁺ T-cells.

Method

In this study, 24 healthy males (age: 24.6?±?3.9 years; weight 83.9?±?10.5 kg; height: 182.4?±?6.2 cm) completed a single bout of endurance (EE) and resistance exercise (RE) in a randomly assigned order on separate days. Blood samples were drawn before (t0), after (t1), and 1 h after (t2) both conditions. T-cell populations, the level of cytoplasmic AhR, and surface PD-1 were assessed by flow cytometry.

Results

T-cell populations changed over time, indicated by an increase in the absolute numbers of CD3⁺ lymphocytes after EE (p?+ CD8⁺ T-cells after EE (p?=?.021). Proportions of T-cell populations changed only after EE (t0-t2: p?=?.029; t1-t2: p?=?.006). The level of cytoplasmic AhR decreased immediately after exercise in both exercise conditions (EE: p?=?.009; RE: p?=?.036). The level of surface PD-1 decreased 1 h after EE (p?=?.005).

Conclusion

We analyzed the level of surface PD-1 and cytoplasmic AhR following acute physical exercise for the first time. Especially EE was observed to impact both AhR and PD-1 levels, undermining its role as the AhR-PD-1 axis modulator. These results provide new insights into the impact of exercise on AhR-signaling, which could potentially be relevant for various chronic diseases.

SUBMITTER: Schenk A

PROVIDER: S-EPMC7862188 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Acute exercise impacts AhR and PD-1 levels of CD8<sup>+</sup> T-cells-Exploratory results from a randomized cross-over trial comparing endurance versus resistance exercise.

Schenk Alexander A Joisten Niklas N Walzik David D Koliamitra Christina C Schoser Daria D Bloch Wilhelm W Zimmer Philipp P

European journal of applied physiology 20201119 2

<h4>Purpose</h4>The programmed cell death protein 1 (PD-1) has become a promising target in cancer immunotherapy. PD-1 expression of CD8<sup>+</sup> T-cells may be increased via the exploitation of aryl hydrocarbon receptor (AhR) signaling with kynurenine (KYN) as a ligand. Since exercise affects KYN metabolism, we exploratory investigated the influence of acute exercise bouts on AhR and PD-1 levels of CD8<sup>+</sup> T-cells.<h4>Method</h4>In this study, 24 healthy males (age: 24.6 ± 3.9 years; ...[more]

PMID: 33211154

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Dataset Information

Acute exercise impacts AhR and PD-1 levels of CD8⁺ T-cells-Exploratory results from a randomized cross-over trial comparing endurance versus resistance exercise.

Purpose

Method

Results

Conclusion

Publications

Acute exercise impacts AhR and PD-1 levels of CD8<sup>+</sup> T-cells-Exploratory results from a randomized cross-over trial comparing endurance versus resistance exercise.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Dataset Information

Acute exercise impacts AhR and PD-1 levels of CD8+ T-cells-Exploratory results from a randomized cross-over trial comparing endurance versus resistance exercise.

Purpose

Method

Results

Conclusion

Publications

Acute exercise impacts AhR and PD-1 levels of CD8<sup>+</sup> T-cells-Exploratory results from a randomized cross-over trial comparing endurance versus resistance exercise.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Acute exercise impacts AhR and PD-1 levels of CD8⁺ T-cells-Exploratory results from a randomized cross-over trial comparing endurance versus resistance exercise.