Ontology highlight
ABSTRACT:
SUBMITTER: Ellis NA
PROVIDER: S-EPMC7862264 | biostudies-literature | 2021 Feb
REPOSITORIES: biostudies-literature
Ellis Nicole A NA Kim Byoungkwan B Tung Jessica J Machner Matthias P MP
Communications biology 20210204 1
Catalytically inactive dCas9 imposes transcriptional gene repression by sterically precluding RNA polymerase activity at a given gene to which it was directed by CRISPR (cr)RNAs. This gene silencing technology, known as CRISPR interference (CRISPRi), has been employed in various bacterial species to interrogate genes, mostly individually or in pairs. Here, we developed a multiplex CRISPRi platform in the pathogen Legionella pneumophila capable of silencing up to ten genes simultaneously. Constra ...[more]