Ontology highlight
ABSTRACT:
SUBMITTER: Nishiyama K
PROVIDER: S-EPMC7864621 | biostudies-literature | 2021 Feb
REPOSITORIES: biostudies-literature
Nishiyama Keine K Takeda Yohei Y Takahashi Kazuki K Fukuyama Mao M Maeki Masatoshi M Ishida Akihiko A Tani Hirofumi H Shigemura Koji K Hibara Akihide A Ogawa Haruko H Tokeshi Manabu M
Analytical and bioanalytical chemistry 20210205 18
Nowadays, the diagnosis of viral infections is receiving broad attention. We have developed a non-competitive fluorescence polarization immunoassay (NC-FPIA), which is a separation-free immunoassay, for a virus detection. H5 subtype avian influenza virus (H5-AIV) was used as a model virus for the proof of concept. The fluorescein-labeled Fab fragment that binds to H5 hemagglutinin was used for NC-FPIA. The purified H5-AIV which has H5 hemagglutinin was mixed with the fluorescein-labeled Fab frag ...[more]