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Isolation of Bacteria with Potential Plant-Promoting Traits and Optimization of Their Growth Conditions.

ABSTRACT: This research aimed at investigating the isolation and identification of bacterial strains with biological nitrogen-fixing capability and phosphate, potassium, and zinc solubilization activities from a durum wheat field under two different tillage practices including 10 years of conventional tillage (CT) and no-tillage (NT) practices. Attempts were also extended to estimate their relative abundances in the soil as well as to develop accurate mathematical models in determining the effect of different temperatures, NaCl concentrations and pH on the growth, and activity of selected isolates. Twelve effective bacterial strains, including Pseudomonas, Acinetobacter, and Comamonas genera, were identified with a great potential to solubilize the insoluble forms of phosphate (from 11.1 to 115.5 mg l^-1 at pH 8), potassium (from 32.2 to 35.6 mg l^-1 at pH 7), and zinc (from 1.11 to 389.90 mg l^-1 at pH 9) as well as to fix N₂ gas (from 19.9 to 25.2 mg l^-1). To our knowledge, this is the first report of the ability of Comamonas testosteroni and Acinetobacter pittii to fix nitrogen and to solubilize insoluble potassium compound, respectively. Three families, Moraxellaceae, Pseudomonadaceae, and Comamonadaceae, showed a higher percentage of abundance in the NT samples as compared to the CT, but only significant difference was observed in the relative abundance of Pseudomonadaceae (P?

SUBMITTER: Yaghoubi Khanghahi M

PROVIDER: S-EPMC7864805 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature

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Isolation of Bacteria with Potential Plant-Promoting Traits and Optimization of Their Growth Conditions.

Yaghoubi Khanghahi Mohammad M Strafella Sabrina S Allegretta Ignazio I Crecchio Carmine C

Current microbiology 20201223 2

This research aimed at investigating the isolation and identification of bacterial strains with biological nitrogen-fixing capability and phosphate, potassium, and zinc solubilization activities from a durum wheat field under two different tillage practices including 10 years of conventional tillage (CT) and no-tillage (NT) practices. Attempts were also extended to estimate their relative abundances in the soil as well as to develop accurate mathematical models in determining the effect of diffe ...[more]

PMID: 33354746

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Project description:Paenibacillus polymyxa is a plant growth-promoting rhizobacterium that has immense potential to be used as an environmentally friendly replacement of chemical fertilizers and pesticides. In the present study, Paenibacillus polymyxa SK1 was isolated from bulbs of Lilium lancifolium. The isolated endophytic strain showed antifungal activities against important plant pathogens like Botryosphaeria dothidea, Fusarium oxysporum, Botrytis cinerea, and Fusarium fujikuroi. The highest percentage of growth inhibition, i.e., 66.67?±?2.23%, was observed for SK1 against Botryosphaeria dothidea followed by 61.19?±?3.12%, 60.71?±?3.53%, and 55.54?±?2.89% against Botrytis cinerea, Fusarium fujikuroi, and Fusarium oxysporum, respectively. The metabolite profiling of ethyl acetate fraction was assessed through the UHPLC-LTQ-IT-MS/MS analysis, and putative identification was done with the aid of the GNPS molecular networking workflow. A total of 29 compounds were putatively identified which included dipeptides, tripeptides, cyclopeptides (cyclo-(Leu-Leu), cyclo(Pro-Phe)), 2-heptyl-3-hydroxy 4-quinolone, 6-oxocativic acid, anhydrobrazilic acid, 1-(5-methoxy-1H-indol-3-yl)-2-piperidin-1-ylethane-1,2-dione, octadecenoic acid, pyochelin, 15-hydroxy-5Z,8Z,11Z, 13E-eicosatetraenoic acid, (Z)-7-[(2R,3S)-3-[(2Z,5E)-Undeca-2,5-dienyl]oxiran-2-yl]hept-5-enoic acid, arginylasparagine, cholic acid, sphinganine, elaidic acid, gossypin, L-carnosine, tetrodotoxin, and ursodiol. The high antifungal activity of SK1 might be attributed to the presence of these bioactive compounds. The isolated strain SK1 showed plant growth-promoting traits such as the production of organic acids, ACC deaminase, indole-3-acetic acid (IAA), siderophores, nitrogen fixation, and phosphate solubilization. IAA production was strongly correlated with the application of exogenous tryptophan concentrations in the medium. Furthermore, inoculation of SK1 enhanced plant growth of two Lilium varieties, Tresor and White Heaven, under greenhouse condition. In the light of these findings, the P. polymyxa SK1 may be utilized as a source of plant growth promotion and disease control in sustainable agriculture.

Project description:BackgroundA diverse community of microbes naturally exists on the phylloplane, the surface of leaves. It is one of the most prevalent microbial habitats on earth and bacteria are the most abundant members, living in communities that are highly dynamic. Today, one of the key challenges for microbiologists is to develop strategies to culture the vast diversity of microorganisms that have been detected in metagenomic surveys.ResultsWe isolated bacteria from the phylloplane of Hedera helix (common ivy), a widespread evergreen, using five growth media: Luria-Bertani (LB), LB01, yeast extract-mannitol (YMA), yeast extract-flour (YFlour), and YEx. We also included a comparison with the uncultured phylloplane, which we showed to be dominated by Proteobacteria, Actinobacteria, Bacteroidetes, and Firmicutes. Inter-sample (beta) diversity shifted from LB and LB01 containing the highest amount of resources to YEx, YMA, and YFlour which are more selective. All growth media equally favoured Actinobacteria and Gammaproteobacteria, whereas Bacteroidetes could only be found on LB01, YEx, and YMA. LB and LB01 favoured Firmicutes and YFlour was most selective for Betaproteobacteria. At the genus level, LB favoured the growth of Bacillus and Stenotrophomonas, while YFlour was most selective for Burkholderia and Curtobacterium. The in vitro plant growth promotion (PGP) profile of 200 isolates obtained in this study indicates that previously uncultured bacteria from the phylloplane may have potential applications in phytoremediation and other plant-based biotechnologies.ConclusionsThis study gives first insights into the total bacterial community of the H. helix phylloplane, including an evaluation of its culturability using five different growth media. We further provide a collection of 200 bacterial isolates underrepresented in current databases, including the characterization of PGP profiles. Here we highlight the potential of simple strategies to obtain higher microbial diversity from environmental samples and the use of high-throughput sequencing to guide isolate selection from a variety of growth media.

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Isolation of Bacteria with Potential Plant-Promoting Traits and Optimization of Their Growth Conditions.

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Isolation of Bacteria with Potential Plant-Promoting Traits and Optimization of Their Growth Conditions.

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