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Enzyme mediated synthesis of hybrid polyedric gold nanoparticles.

ABSTRACT: Large protein complexes carry out some of the most complex activities in biology^1,2. Such structures are often assembled spontaneously through the process of self-assembly and have characteristic chemical or biological assets in the cellular mechanisms³. Gold-based nanomaterials have attracted much attention in many areas of chemistry, physics and biosciences because of their size- and shape-dependent optic, electric, and catalytic properties. Here we report for the first time a one step synthesis in which Manganese Superoxide Dismutase protein plays a key role in the reduction of gold salts via the use of a Good's buffer (HEPES) to produce gold nanoparticles, compared to other proteins as catalase (CAT) and bovine serum albumin (BSA).We prove that this effect is directly related with the biological activities of the proteins that have an effect on the gold reduction mechanisms. Such synthesis route also induces the integration of proteins directly in the AuNPs that are intrinsically safe by design using a one-step production method. This is an important finding that will have uses in various applications, particularly in the green synthesis of novel nanomaterials.

SUBMITTER: Arib C

PROVIDER: S-EPMC7864913 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature

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Enzyme mediated synthesis of hybrid polyedric gold nanoparticles.

Arib Célia C Spadavecchia Jolanda J de la Chapelle Marc Lamy ML

Scientific reports 20210205 1

Large protein complexes carry out some of the most complex activities in biology<sup>1,2</sup>. Such structures are often assembled spontaneously through the process of self-assembly and have characteristic chemical or biological assets in the cellular mechanisms<sup>3</sup>. Gold-based nanomaterials have attracted much attention in many areas of chemistry, physics and biosciences because of their size- and shape-dependent optic, electric, and catalytic properties. Here we report for the first t ...[more]

PMID: 33547353

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Project description:Pseudomonas aeruginosa was used to synthesize anisotropic gold nanoparticles from the unusually reducible aryldiazonium gold (III) salt of the chemical formula [HOOC-4-C6H4N≡N]AuCl4 (abbreviated as DS-AuCl4). We investigated the effect of bacterial cell density, temperature, and pH on the AuNP synthesis. The bacterial cell density of 6.0 × 108 CFU/mL successfully reduced 0.5 mM DS-AuCl4 salt to AuNPs after incubation at 37 °C (24 h), 42 °C (24 h), and 25 °C (48 h). Transmission electron microscopy (TEM) images revealed the formation of spherical, triangle, star, hexagon, and truncated triangular morphologies for the AuNPs synthesized using P. aeruginosa bacteria. The average size of AuNPs synthesized at 25 °C (48 h), 37 °C (24 h), and 42 °C (24 h) was 39.0 ± 9.1 nm, 26.0 ± 8.1 nm, and 36.7 ± 7.7 nm, respectively. The average size of AuNPs synthesized at pH 3.7, 7.0, and 12.7 was 36.7 ± 7.7 nm, 14.7 ± 3.8 nm, and 7.3 ± 2.5 nm, respectively, with the average size decreasing at a pH of 12.7. The reduction of the DS-AuCl4 salt was confirmed using X-ray photoelectron spectroscopy (XPS). The significant peaks for C1s, Au4f doublet, N1s, and O1s are centered at 285, 84-88, 400, and 532 eV. The ability of inactivated bacteria (autoclave-dead and mechanically lysed bacteria), peptidoglycan, and lipopolysaccharides to reduce the DS-AuCl4 salt to AuNPs was also investigated. Anisotropic AuNPs were synthesized using inactivated bacteria and peptidoglycan but not using lipopolysaccharides. The AuNPs demonstrated biocompatibility with human RBCs and were safe, with no antibacterial activities against Escherichia coli and Staphylococcus aureus. This is the first report demonstrating the synthesis of AuNPs using aryldiazonium gold(III) salts with P. aeruginosa. These AuNPs are promising candidates for exploring potential applications in nanomedicine and drug delivery. KEY POINTS: • Anisotropic AuNPs were synthesized using P. aeruginosa bacteria. • Dead and lysed bacterial residues synthesized anisotropic AuNPs. • AuNPs are hemocompatible.

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Enzyme mediated synthesis of hybrid polyedric gold nanoparticles.

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Enzyme mediated synthesis of hybrid polyedric gold nanoparticles.

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