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Quantification of Immune Variables from Liquid Biopsy in Breast Cancer Patients Links V?2⁺ ?? T Cell Alterations with Lymph Node Invasion.

ABSTRACT: The rationale for therapeutic targeting of V?2⁺ ?? T cells in breast cancer is strongly supported by in vitro and murine preclinical investigations, characterizing them as potent breast tumor cell killers and source of Th1-related cytokines, backing cytotoxic ?? T cells. Nonetheless, insights regarding V?2⁺ ?? T cell phenotypic alterations in human breast cancers are still lacking. This paucity of information is partly due to the challenging scarcity of these cells in surgical specimens. ?? T cell phenotypic alterations occurring in the tumor bed are detectable in the periphery and correlate with adverse clinical outcomes. Thus, we sought to determine through an exploratory study whether V?2⁺ ?? T cells phenotypic changes can be detected within breast cancer patients' peripheral blood, along with association with tumor progression. By using mass cytometry, we quantified 130 immune variables from untreated breast cancer patients' peripheral blood. Supervised analyses and dimensionality reduction algorithms evidenced circulating V?2⁺ ?? T cell phenotypic alterations already established at diagnosis. Foremost, terminally differentiated V?2⁺ ?? T cells displaying phenotypes of exhausted senescent T cells associated with lymph node involvement. Thereby, our results support V?2⁺ ?? T cells implication in breast cancer pathogenesis and progression, besides shedding light on liquid biopsies to monitor surrogate markers of tumor-infiltrating V?2⁺ ?? T cell antitumor activity.

SUBMITTER: Fattori S

PROVIDER: S-EPMC7865589 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature

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Quantification of Immune Variables from Liquid Biopsy in Breast Cancer Patients Links Vδ2+ γδ T Cell Alterations with Lymph Node Invasion.

Fattori Stéphane S Gorvel Laurent L Granjeaud Samuel S Rochigneux Philippe P Rouvière Marie-Sarah MS Ben Amara Amira A Boucherit Nicolas N Paul Magali M Dauplat Marie Mélanie MM Thomassin-Piana Jeanne J Paciencia-Gros Maria M Avenin Morgan M Pakradouni Jihane J Barrou Julien J Charafe-Jauffret Emmanuelle E Houvenaeghel Gilles G Lambaudie Eric E Bertucci François F Goncalves Anthony A Tarpin Carole C Nunès Jacques A JA Devillier Raynier R Chretien Anne-Sophie AS Olive Daniel D

Cancers 20210125 3

The rationale for therapeutic targeting of Vδ2+ γδ T cells in breast cancer is strongly supported by in vitro and murine preclinical investigations, characterizing them as potent breast tumor cell killers and source of Th1-related cytokines, backing cytotoxic αβ T cells. Nonetheless, insights regarding Vδ2+ γδ T cell phenotypic alterations in human breast cancers are still lacking. This paucity of information is partly due to the challenging scarcity of these cells in surgi ...[more]

PMID: 33503843

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Project description:To limit the potential short and long-term morbidity of lymphadenectomy, sentinel lymph node biopsy has been proposed for endometrial cancer. The principle of sentinel lymph node biopsy relies on removal of a small number of lymph nodes that are the first drainage basins from a tumor and thus the most likely to harbor tumor cells. While the procedure may reduce morbidity, efficacy data are limited and little is known about how commonly the procedure is performed.We examined the patterns and predictors of use of sentinel lymph node biopsy and outcomes of the procedure in women with endometrial cancer who underwent hysterectomy.We used the Perspective database to identify women with uterine cancer who underwent hysterectomy from 2011 through 2015. Billing and charge codes were used to classify women as having undergone lymphadenectomy, sentinel lymph node biopsy, or no nodal assessment. Multivariable models were used to examine clinical, demographic, and hospital characteristics with use of sentinel lymph node biopsy. Length of stay and cost were compared among the different methods of nodal assessment.Among 28,362 patients, 9327 (32.9%) did not undergo nodal assessment, 17,669 (62.3%) underwent lymphadenectomy, and 1366 (4.8%) underwent sentinel lymph node biopsy. Sentinel lymph node biopsy was performed in 1.3% (95% confidence interval, 1.0-1.6%) of abdominal hysterectomies, 3.4% (95% confidence interval, 2.7-4.1%) of laparoscopic hysterectomies, and 7.5% (95% confidence interval, 7.0-8.0%) of robotic-assisted hysterectomies. In a multivariable model, more recent year of surgery was associated with performance of sentinel lymph node biopsy. Compared to abdominal hysterectomy, those undergoing laparoscopic (adjusted risk ratio, 2.45; 95% confidence interval, 1.89-3.18) and robotic-assisted (adjusted risk ratio, 2.69; 95% confidence interval, 2.19-3.30) hysterectomy were more likely to undergo sentinel lymph node biopsy. Among women who underwent minimally invasive hysterectomy, length of stay and cost were lower for sentinel lymph node biopsy compared to lymphadenectomy.The use of sentinel lymph node biopsy for endometrial cancer increased from 2011 through 2015. The increased use was most notable in women who underwent a robotic-assisted hysterectomy.

Dataset Information

Quantification of Immune Variables from Liquid Biopsy in Breast Cancer Patients Links V?2⁺ ?? T Cell Alterations with Lymph Node Invasion.

Publications

Quantification of Immune Variables from Liquid Biopsy in Breast Cancer Patients Links Vδ2<sup>+</sup> γδ T Cell Alterations with Lymph Node Invasion.

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OmicsDI is part of the ELIXIR infrastructure

Dataset Information

Quantification of Immune Variables from Liquid Biopsy in Breast Cancer Patients Links V?2+ ?? T Cell Alterations with Lymph Node Invasion.

Publications

Quantification of Immune Variables from Liquid Biopsy in Breast Cancer Patients Links Vδ2<sup>+</sup> γδ T Cell Alterations with Lymph Node Invasion.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Quantification of Immune Variables from Liquid Biopsy in Breast Cancer Patients Links V?2⁺ ?? T Cell Alterations with Lymph Node Invasion.