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Beneficial effect and mechanism of walnut oligopeptide on Lactobacillus plantarum Z7.

ABSTRACT: Prebiotics can stimulate the growth and activity of probiotics and have a variety of physiological functions. However, the study of walnut oligopeptides as prebiotics to promote probiotics is rarely reported. Therefore, in order to explore the beneficial effect of walnut oligopeptide (WOPs) on Lactobacillus plantarum Z7, WOPs was added to the medium of L. plantarum Z7, and the utilization of WOPs, the effect of WOPs on the biofilm, extracellular polymeric substances, and bacterial death were explored. The results showed that the growth-promoting effect was strengthened with the increasing concentrations of WOPs. The content of bacterial biofilm and EPS increased significantly, and the number of dead bacteria decreased. The beneficial effect of WOPs was probably because that it enhanced the secretion of biofilm which was regulated by bacterial quorum sensing system and promoted the ability of bacteria to resist the adverse environment, thus promoting the growth and reproduction of bacteria.

SUBMITTER: Ding T

PROVIDER: S-EPMC7866578 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature

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Beneficial effect and mechanism of walnut oligopeptide on Lactobacillus plantarum Z7.

Ding Ting T Li Yong Y

Food science & nutrition 20210108 2

Prebiotics can stimulate the growth and activity of probiotics and have a variety of physiological functions. However, the study of walnut oligopeptides as prebiotics to promote probiotics is rarely reported. Therefore, in order to explore the beneficial effect of walnut oligopeptide (WOPs) on Lactobacillus plantarum Z7, WOPs was added to the medium of L. plantarum Z7, and the utilization of WOPs, the effect of WOPs on the biofilm, extracellular polymeric substances, and bacterial ...[more]

PMID: 33598152

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Project description:Heat stress resistance and response were studied in strains of Lactobacillus plantarum. Stationary-phase cells of L. plantarum DPC2739 had decimal reduction times (D values) (D value was the time that it took to reduce the number of cells by 1 log cycle) in sterile milk of 32.9, 14.7, and 7.14 s at 60, 72, and 75 degrees C, respectively. When mid-exponential-phase cells were used, the D values decreased. The temperature increases which caused a 10-fold reduction in the D value ranged from 9 to 20 degrees C, depending on the strain. Part of the cell population treated at 72 degrees C for 90 s recovered viability during incubation at 7 degrees C in sterile milk for 20 days. When mid-exponential- or stationary-phase cells of L. plantarum DPC2739 were adapted to 42 degrees C for 1 h, the heat resistance at 72 degrees C for 90 s increased ca. 3 and 2 log cycles, respectively. Heat-adapted cells also showed increased growth at pH 5 and in the presence of 6% NaCl. Two-dimensional gel electrophoresis of proteins expressed by control and heat-adapted cells revealed changes in the levels of expression of 31 and 18 proteins in mid-exponential- and stationary-phase cells, respectively. Twelve proteins were commonly induced. Nine proteins induced in the heat-adapted mid-exponential- and/or stationary-phase cells of L. plantarum DPC2739 were subjected to N-terminal sequencing. These proteins were identified as DnaK, GroEL, trigger factor, ribosomal proteins L1, L11, L31, and S6, DNA-binding protein II HlbA, and CspC. All of these proteins have been found to play a role in the mechanisms of stress adaptation in other bacteria. Antibodies against GroES detected a protein which was induced moderately, while antibodies against DnaJ and GrpE reacted with proteins whose level of expression did not vary after heat adaptation. This study showed that the heat resistance of L. plantarum is a complex process involving proteins with various roles in cell physiology, including chaperone activity, ribosome stability, stringent response mediation, temperature sensing, and control of ribosomal function. The physiological mechanisms of response to pasteurization in L. plantarum are fundamental for survival in cheese during manufacture.

Dataset Information

Beneficial effect and mechanism of walnut oligopeptide on Lactobacillus plantarum Z7.

Publications

Beneficial effect and mechanism of walnut oligopeptide on <i>Lactobacillus plantarum</i> Z7.

Similar Datasets

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