Unknown

Dataset Information

0

Colorectal cancer cell lines show striking diversity of their O-glycome reflecting the cellular differentiation phenotype.


ABSTRACT: Alterations in protein glycosylation in colorectal cancer (CRC) have been extensively studied using cell lines as models. However, little is known about their O-glycome and the differences in glycan biosynthesis in different cell types. To provide a better understanding of the variation in O-glycosylation phenotypes and their association with other molecular features, an in-depth O-glycosylation analysis of 26 different CRC cell lines was performed. The released O-glycans were analysed on porous graphitized carbon nano-liquid chromatography system coupled to a mass spectrometer via electrospray ionization (PGC-nano-LC-ESI-MS/MS) allowing isomeric separation as well as in-depth structural characterization. Associations between the observed glycan phenotypes with previously reported cell line transcriptome signatures were examined by canonical correlation analysis. Striking differences are observed between the O-glycomes of 26 CRC cell lines. Unsupervized principal component analysis reveals a separation between well-differentiated colon-like and undifferentiated cell lines. Colon-like cell lines are characterized by a prevalence of I-branched and sialyl Lewis x/a epitope carrying glycans, while most undifferentiated cell lines show absence of Lewis epitope expression resulting in dominance of truncated ?2,6-core sialylated glycans. Moreover, the expression of glycan signatures associates with the expression of glycosyltransferases that are involved in their biosynthesis, providing a deeper insight into the regulation of glycan biosynthesis in different cell types. This untargeted in-depth screening of cell line O-glycomes paves the way for future studies exploring the role of glycosylation in CRC development and drug response leading to discovery of novel targets for the development of anti-cancer antibodies.

SUBMITTER: Madunic K 

PROVIDER: S-EPMC7867528 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature

altmetric image

Publications

Colorectal cancer cell lines show striking diversity of their O-glycome reflecting the cellular differentiation phenotype.

Madunić Katarina K   Zhang Tao T   Mayboroda Oleg A OA   Holst Stephanie S   Stavenhagen Kathrin K   Jin Chunsheng C   Karlsson Niclas G NG   Lageveen-Kammeijer Guinevere S M GSM   Wuhrer Manfred M  

Cellular and molecular life sciences : CMLS 20200331 1


Alterations in protein glycosylation in colorectal cancer (CRC) have been extensively studied using cell lines as models. However, little is known about their O-glycome and the differences in glycan biosynthesis in different cell types. To provide a better understanding of the variation in O-glycosylation phenotypes and their association with other molecular features, an in-depth O-glycosylation analysis of 26 different CRC cell lines was performed. The released O-glycans were analysed on porous  ...[more]

Similar Datasets

| S-EPMC9157004 | biostudies-literature
| S-EPMC3822923 | biostudies-literature
| S-EPMC6678350 | biostudies-literature
| S-EPMC10003090 | biostudies-literature
| S-EPMC5860729 | biostudies-literature
| S-EPMC7226743 | biostudies-literature
| S-EPMC6039343 | biostudies-literature
| S-EPMC8631502 | biostudies-literature
| S-EPMC6078140 | biostudies-other
| S-EPMC9221004 | biostudies-literature