A practical guide to cancer subclonal reconstruction from DNA sequencing.
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ABSTRACT:
SUBMITTER: Tarabichi M
PROVIDER: S-EPMC7867630 | biostudies-literature | 2021 Feb
REPOSITORIES: biostudies-literature
Publications
A practical guide to cancer subclonal reconstruction from DNA sequencing.
Tarabichi Maxime M Salcedo Adriana A Deshwar Amit G AG Ni Leathlobhair Máire M Wintersinger Jeff J Wedge David C DC Van Loo Peter P Morris Quaid D QD Boutros Paul C PC
Nature methods 20210104 2
Subclonal reconstruction from bulk tumor DNA sequencing has become a pillar of cancer evolution studies, providing insight into the clonality and relative ordering of mutations and mutational processes. We provide an outline of the complex computational approaches used for subclonal reconstruction from single and multiple tumor samples. We identify the underlying assumptions and uncertainties in each step and suggest best practices for analysis and quality assessment. This guide provides a pragm ...[more]
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