Protein-protein interaction analysis reveals a novel cancer stem cell related target TMEM17 in colorectal cancer.

ABSTRACT:

Background

Cancer stem cells (CSCs) are a small subpopulation of cells within tumors with stem cell property. Increased evidence suggest that CSCs could be responsible for chemoresistance and recurrence in colorectal cancer (CRC). However, a reliable therapeutic target on CSCs is still lacking.

Methods

Here we describe a two-step strategy to generate CSC targets with high selectivity for colon stem cell markers, specific proteins that are interacted with CSC markers were selected and subsequently validated in a survival analysis. TMEM17 protein was found and its biological functions in CRC cells were further examined. Finally, we utilized the Gene Set Enrichment Analysis (GSEA) to investigate the potential mechanisms of TMEM17 in CRC.

Results

By combining protein-prot

SUBMITTER: Yu ZL 

PROVIDER: S-EPMC7868027 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature