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Targeting NF-?B mediated cell signaling pathway and inflammatory mediators by 1,2-diazole in A549 cells in vitro.

ABSTRACT: Lung cancer is the leading cause of cancer deaths globally. The objective of this study was to investigate the effect of 1,2-diazole (pyrazole) as an anti-cancer drug on human non-small cell lung carcinoma A549 cells. We attempt to examine the expression level of pro-inflammatory proteins such as TNF-?, NF-?B-p65, MMP-2 and E-Cadherin which are commonly associated with an inflammatory response in epithelial cells and apoptosis in A549 cells. The LPS-induced cytokines and inflammatory mediators include TNF-?, IL-6, iNOS and COX-2 levels in A549 cells and the effect of pyrazole was studied. The present study reveals that, pyrazole inhibits A549 cells by suppressing TNF-? induced MMP-2 expression, thereby inhibiting the nuclear translocation of NF-?B-p65. Pyrazole significantly up-regulate the E-cadherin level and down-regulated MMP-2 expression that could probably preventing A549 cancer cells to invade. The study further substantiated the anti-cancer property of pyrazole by regulating the above mentioned level of LPS-induced cytokines and inflammatory mediators. The observations of the present study open a possibility for the development of an effective therapeutic agent that targets inflammatory and signaling pathway mediators to challenge human non-small cell lung carcinoma.

SUBMITTER: Vinod Prabhu V 

PROVIDER: S-EPMC7868824 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature

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Targeting NF-κB mediated cell signaling pathway and inflammatory mediators by 1,2-diazole in A549 cells <i>in vitro</i>.

Vinod Prabhu Venugopal V   Elangovan Perumal P   Niranjali Devaraj Sivasithambaram S   Sakthivel Kunnathur Murugesan KM  

Biotechnology reports (Amsterdam, Netherlands) 20210128

Lung cancer is the leading cause of cancer deaths globally. The objective of this study was to investigate the effect of 1,2-diazole (pyrazole) as an anti-cancer drug on human non-small cell lung carcinoma A549 cells. We attempt to examine the expression level of pro-inflammatory proteins such as TNF-α, NF-κB-p65, MMP-2 and E-Cadherin which are commonly associated with an inflammatory response in epithelial cells and apoptosis in A549 cells. The LPS-induced cytokines and inflammatory mediators i  ...[more]

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